- ￥5800
- Proteintech
- 84469-5-PBS
- 2025年10月09日
- IF/ICC, Indirect ELISA
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
IF/ICC, Indirect ELISA
- 靶点：
Tie-2/CD202b
- 抗体英文名：
Mouse Tie-2/CD202b Recombinant monoclonal antibody, PBS Only
- 抗体名：
Mouse Tie-2/CD202b Recombinant monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
84469-5-PBS targets Tie-2/CD202b in IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | IF/ICC, Indirect ELISA |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg1514 Product name: Recombinant Mouse Tie-2/CD202b protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 23-744 aa of NM_001290549.1 Sequence: AMDLILINSLPLVSDAETSLTCIASGWHPHEPITIGRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGQAIRIRTMKMRQQASFLPATLTMTVDRGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPDCSRPCTTCKNNGVCHEDTGECICPPGFMGRTCEKACEPHTFGRTCKERCSGPEGCKSYVFCLPDPYGCSCATGWRGLQCNEACPSGYYGPDCKLRCHCTNEEICDRFQGCLCSQGWQGLQCEKEGRPRMTPQIEDLPDHIEVNSGKFNPICKASGWPLPTSEEMTLVKPDGTVLQPNDFNYTDRFSVAIFTVNRVLPPDSGVWVCSVNTVAGMVEKPFNISVKVLPEPLHAPNVIDTGHNFAIINISSEPYFGDGPIKSKKLFYKPVNQAWKYIEVTNEIFTLNYLEPRTDYELCVQLARPGEGGEGHPGPVRRFTTASIGLPPPRGLSLLPKSQTALNLTWQPIFTNSEDEFYVEVERRSLQTTSDQQNIKVPGNLTSVLLSNLVPREQYTVRARVNTKAQGEWSEELRAWTLSDILPPQPENIKISNITDSTAMVSWTIVDGYSISSIIIRYKVQGKNEDQHIDVKIKNATVTQYQLKGLEPETTYHVDIFAENNIGSSNPAFSHELRTLPHSPASADLGGGK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | endothelial-specific receptor tyrosine kinase |
| 别名 | 241753F12, Angiopoietin-1 receptor, CD202b, EC:2.7.10.1, Endothelial tyrosine kinase |
| 计算分子量 | 126kDa |
| GenBank蛋白编号 | NM_001290549.1 |
| 基因名称 | Tek |
| Gene ID (NCBI) | 21687 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q02858 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Tie-2, or TEK, is a receptor tyrosine kinase (RTK) specifically expressed in endothelial cells. It plays a crucial role in angiogenesis, forming new blood vessels, and maintaining vascular stability. Tie-2 is a member of the Tie receptor family, characterized by the presence of immunoglobulin and epidermal growth factor (EGF) homology domains. Tie-2 plays a significant role in several vascular diseases, including vascular malformations like venous malformations. It is also implicated in pathological vascular remodeling associated with tumor growth, where it is often overexpressed in tumor vessels.
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文献和实验液，加入6ml DMEM完全培养基，37℃孵育过夜，再换液。 7、培养过程中观察细胞生长情况。约2周后可观察到细胞病变（CPE）出现（如用pAdTrack-CMV质粒，由于含GFP,可观察到绿色荧光）。四、重组病毒鉴定 1、转导10-14d后，收集细胞沉淀，加入2ml灭菌的PBS混悬，冻融细胞，离心后收集上清保存于-80℃。 2、取30-50% 步骤1中上清，感染50-70%饱和度的25cm2方瓶中293细胞。2-3d后出现明显细胞病变。 3、感染后3-5d，当1/3-1/2细胞漂浮时
-DNA液，加入6ml DMEM完全培养基，37℃孵育过夜，再换液。 7 培养过程中观察细胞生长情况。约2周后可观察到细胞病变（CPE）出现（如用pAdTrack-CMV质粒，由于含GFP，可观察到绿色荧光）。 四 重组病毒鉴定 1 转导10-14d后，收集细胞沉淀，加入2ml灭菌的PBS混悬，冻融细胞，离心后收集上清保存于-80℃。 2 取30-50% 步骤1中上清，感染50-70%饱和度的25cm2 方瓶中293细胞。2-3d后出现明显细胞
/ml denatured rat tail collagen (Vitrogen; Collagen Corporation). To prepare collagen-coated dishes: Dilute Vitrogen stock (~3 mg/ml) 1:100 in sterile PBS, then completely cover the surface of a 10cm dish with 3-5 ml. Incubate at 33
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