- ￥2800
- Proteintech
- CL488-66154
- 2025年10月09日
- IF-P
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human CFB protein
- 亚型：
IgM
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
IF-P
- 靶点：
Complement factor B
- 抗体英文名：
CoraLite® Plus 488-conjugated Complement factor B Monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated Complement factor B Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF-P detected in | human liver tissue |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66154 targets Complement factor B in IF-P applications and shows reactivity with human samples.
| 经测试应用 | IF-P |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag19107 Product name: Recombinant human CFB protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 574-762 aa of BC007990 Sequence: DYDVALIKLKNKLKYGQTIRPICLPCTEGTTRALRLPPTTTCQQQKEELLPAQDIKALFVSEEEKKLTRKEVYIKNGDKKGSCERDAQYAPGYDKVKDISEVVTPRFLCTGGVSPYADPNTCRGDSGGPLIVHKRSRFIQVGVISWGVVDVCKNQKRQKQVPAHARDFHINLFQVLPWLKEKLQDEDLG |
| 宿主/亚型 | Mouse / IgM |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | complement factor B |
| 别名 | BF, BFD, C3/C5 convertase, CFAB, CFB, complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B |
| 计算分子量 | 86 kDa |
| 观测分子量 | 93 kDa |
| GenBank蛋白编号 | BC007990 |
| 基因名称 | Complement factor B |
| Gene ID (NCBI) | 629 |
| RRID | AB_2934449 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Thiophilic affinity chromatograph |
| UNIPROT ID | P00751 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is cleaved into Ba and Bb by factor D in the presence of C3b. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.
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文献和实验Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
(Bio-Rad). The cell lysates were sonicated (5 s, Sonifier® B-12, Branson Ultrasonics Corp), spun, and mixed with 3× sodium dodecyl sulfate (SDS) loading buffer (240 mM Tris�HCl pH 6.8, 6% SDS, 0.02% bromphenol blue, 30% glycerol, 3% β-mercaptoethanol
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色（可选） 1. 将细胞重悬在 0.5 ml DNA 染色液中（例如 DRAQ5® #4084）。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗： 抗兔： Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4412 Anti-Rabbit IgG（H+L）， F（ab
CD55 分子 CD55 常用单克隆抗体或代号： 143- 30,BRIC110,BRIC123;(DAF) 主要表达细胞： 广泛 [NL] 分子质量(kDa)和结构： gp70(GPI连接) 功 能： 衰变加速因子,调节补体活化 CD55 Aka decay accelerating factor Binds C3bBb (alternative pathway
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