- ￥600 - 1200
- Proteintech
- CL405-98028
- 2025年10月09日
- FC (Intra)
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 405 Fluorescent Dye
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
FC (Intra)
- 靶点：
MCP-1/CCL2
- 抗体英文名：
CoraLite® Plus 405 Anti-Mouse MCP-1/CCL2 Rabbit Recombinant Antibody
- 抗体名：
CoraLite® Plus 405 Anti-Mouse MCP-1/CCL2 Rabbit Recombinant Antibody
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥600.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1200.0 |
经过测试的应用
| Positive FC (Intra) detected in | LPS and Brefeldin A treated RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL405-98028 targets MCP-1/CCL2 in FC (Intra) applications and shows reactivity with mouse samples.
| 经测试应用 | FC (Intra) |
| 经测试反应性 | mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) ligand 2 |
| 别名 | Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2 |
| GenBank蛋白编号 | NM-011333 |
| 基因名称 | MCP-1/CCL2 |
| Gene ID (NCBI) | 20296 |
| RRID | AB_3672412 |
| 偶联类型 | CoraLite® Plus 405 Fluorescent Dye |
| 最大激发/发射波长 | 399 nm / 422 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P10148 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.
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文献和实验ELISA Procedure for Measuring Serum Antibody Titer
of an antibody is shown in Figure 22. The titer was estimated to be 1 in 2400. Figure 22. Antibody titer graph (X = dilution corresponding to Ab titer) Further Methods for Evaluating Anti-peptide Antibodies If the antibodies are to be used as a reagent
请教前辈，我要做westernblot及免疫组化，抗原是人源性的PEDF，实验动物是大鼠，以下有两种santa cruz公司的抗体，source及specificity的说明不同，请问我选用哪一种更为适合，两者有何区别阿？谢谢指教！！！！ SOURCE PEDF (H-125) is a rabbit polyclonal antibody raised against a recombinant protein corresponding to amino acids 281-405
Incubate the plate overnight at 4°C (or for 2-3 hr at 30°C). Wash the plate extensively (3 x 5 min) with 300 µl/well of HBST. Dilute goat anti-rabbit IgG coupled to AP 5,000-10,000
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