- ￥720 - 1500
- Proteintech
- PE-FcA98084
- 2025年10月09日
- FC
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
PE Fluorescent Dye
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
FC
- 靶点：
SELPLG/CD162
- 抗体英文名：
FcZero-rAb™ PE Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody
- 抗体名：
FcZero-rAb™ PE Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody
- 规格：
25tests/100tests
| 规格： | 25tests | 产品价格： | ￥720.0 |
|---|---|---|---|
| 规格： | 100tests | 产品价格： | ￥1500.0 |
经过测试的应用
| Positive FC detected in | human peripheral blood leukocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-FcA98084 targets SELPLG/CD162 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | selectin P ligand |
| 别名 | SELPLG, PSGL1, PSGL 1, P-selectin glycoprotein ligand 1, CLA |
| 计算分子量 | 412 aa, 43 kDa |
| GenBank蛋白编号 | BC029782 |
| 基因名称 | SELPLG |
| Gene ID (NCBI) | 6404 |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q14242 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
干货 | CUT and Tag 让 ChIP-Seq更简单高效！
说明书推荐的反应体系进行 CUT&Tag 实验；其中，pG-Tn5 转座子使用的终浓度为 0.04μM ，一抗为 H3K27me3（CST，#9733），二抗为 Goat anti Rabbit（Bioworld，#BS13271）。 A、文库峰型检测 使用 Agilent 2100 Bioanalyzer 进行文库分布检测。 图 2. 使用 TD901 构建 100 cells CUT&Tag 文库峰型图 图 3. 使用 TD901 构建 10,000 cells CUT&Tag
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G
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