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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1
- 保存条件:
Store at -80°C.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Mouse
- 应用范围:
ELISA, Non-Neutralization
- 靶点:
NeutraControl IFN-gamma
- 抗体英文名:
NeutraControl IFN-gamma Monoclonal antibody, PBS Only
- 抗体名:
NeutraControl IFN-gamma Monoclonal antibody, PBS Only
- 规格:
100ug
69507-1-Ig
产品信息
69507-1-PBS targets IFN-gamma in applications and shows reactivity with human samples.
| 经测试应用 | ELISA, Non-Neutralization |
| 经测试反应性 | human |
| 免疫原 | fusion protein |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | IFN gamma |
| 别名 | NeutraControl IFN gamma, IFNG, 2F11G6, IFG, IFN gamma |
| 基因名称 | IFNG |
| Gene ID (NCBI) | 3458 |
| ENSEMBL Gene ID | ENSG00000111537 |
| 偶联类型 | Unconjugated |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P01579 |
| 储存缓冲液 | PBS Only, pH 7.4. |
| 储存 | Store at -80°C. |
背景介绍
Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.
This antibody is a neutralizing control antibody for IFN gamma, the immunogen is the same as Neutrakine 69007-1-Ig but could not neutralize human IFN gamma.
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文献和实验1. 杂交瘤阳性克隆的筛选与克隆化 在HAT培养基中生长的杂交瘤细胞,只有少数是分泌预定特异性单克隆抗体的细胞,因此,必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法,筛选出能产生所需单克隆抗体的阳性杂交瘤细胞,并进行克隆扩增。经过全面鉴定其所分泌单克隆抗体的免疫球蛋白类型、亚类、特异性、亲和力、识别抗原的表位及其分子量后,及时进行冻存。2. 单克隆抗体的大量制备 单克隆抗体的大量制备重要采用动物体内诱生法和体外培养法。
过夜。(5)用少许PBS将交联物全部洗出,收集洗出液,加1/20V体积新鲜配制的5mg/ml NaBH4溶液,混匀,室温作用30分钟;再加入3/20V NaBH4溶液,混匀,室温作用1小时(或4℃过夜)。(6)将交联物过Sephadex G200或Sepharose 6B(2.6×50cm)层析纯化,分管收集第一峰。(7)酶结合物质量鉴定:克分子比值测定酶量(mg/ml)=OD403×0.4IgG量(mg/ml)=(OD280-OD403×0.3)×0.62克分子比值(E/P)=酶量×4/IgG量
一、材料及试剂 1、猪瘟单抗纯化抗原 2、兔抗猪IgG酶标抗体 3、猪瘟阳性血清 4、猪瘟阴性血清 1~4均由指定的生物制品所购买。 5、ELISA反应板 6、包被液 碳酸钠 1.50g 碳酸氢钠 2.93g H2O加至 1 000ml pH值9.6 7、PBS液 氯化钠 8.90g 磷酸二氢钾 0.20g 无水磷酸氢二钠 2.13g 加双馏水 1 000ml 8、PBS-吐温洗涤液 PBS液 1 000ml 犊牛血清 5ml 混合即可 9、底物
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