- ￥2800
- Proteintech
- CL647-13791
- 2025年10月09日
- IF/ICC, FC (Intra)
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Spartin, SPG20 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
CoraLite® Plus 647 Fluorescent Dye
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
Spartin/SPG20
- 抗体英文名：
CoraLite® Plus 647-conjugated Spartin/SPG20 Polyclonal antibody
- 抗体名：
CoraLite® Plus 647-conjugated Spartin/SPG20 Polyclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-13791 targets Spartin/SPG20 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag4815 Product name: Recombinant human Spartin, SPG20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC047083 Sequence: MEQEPQNGEPAEIKIIREAYKKAFLFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSKESEHTGTGWESARQMQQKMKETLQNVRTRLEILEKGLATSLQNDLQEVPKLYPEFPPKDMCEKLPEPQSFSSAPQHAEVNGNTSTPSAGAVAAPASLSLPSQSCPAEAPPAYTPQAAEGHYTVSYGTDSGEFSSVGEEFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTAGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNRAEEENEFQI |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spastic paraplegia 20 (Troyer syndrome) |
| 别名 | SPG20, KIAA0610, SPART, Spartin, SPG 20 |
| 计算分子量 | 72 kDa |
| 观测分子量 | 75 kDa, 84 kDa |
| GenBank蛋白编号 | BC047083 |
| 基因名称 | Spartin |
| Gene ID (NCBI) | 23111 |
| RRID | AB_2920224 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N0X7 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPG20 (spastic paraplegia 20) gene encodes a multifunctional Spartin protein. SPG20 protein is highly expressed in adipose tissue and may be implicated in endosomal trafficking and microtubule dynamics. SPG20 is mutated in Troyer syndrome, a hereditary spastic paraplegia defined by degeneration of upper motor neurons, recent study showed that regulation of SPG20 on mitochondrial calcium homeostasis may contribute to the pathophysiology of Troyer syndrome. Hypermethylation of SPG20 promoter, found in colorectal cancer patients, may play a role in cytokinesis arrest in colorectal tumorigenesis.
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文献和实验：单克隆抗体设立相应亚型的对照，而多克隆抗体需要对应种属的 IgG 作为对照。用血球计数法或是其他方法对细胞计数。 1. 将 0.5-1 x 106 个细胞分到各检测管中（依照体积）。 2. 各管加入 2-3 ml 孵育缓冲液漂洗，离心回收细胞。重复一次。 3. 将每个管中的细胞重悬在 100 µl 孵育缓冲液中。 4. 室温，孵育缓冲液封闭 10 分钟。 5. 按合适的稀释比例在检测管中加入未标记，生物素标记或是荧光素标记的一抗。具体的用量参考抗体各自的说明书。
3268元 CL-Xposure X 光胶片(5”x7”) 34090 Pierce 100
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