- ￥3580
- Proteintech
- G22167-1-5C
- 2025年10月09日
- Single Cell (Intra)
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human IQGAP1 protein
- 亚型：
IgG
- 保存条件：
2-8°C Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
5CFLX
- 适应物种：
Human
- 宿主：
Rabbit
- 应用范围：
Single Cell (Intra)
- 靶点：
IQGAP1
- 抗体英文名：
MultiPro® 5CFLX Anti-Human IQGAP1 (Polyclonal)
- 抗体名：
MultiPro® 5CFLX Anti-Human IQGAP1 (Polyclonal)
- 规格：
10ug
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G22167-1-5C targets IQGAP1 in Single Cell (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | Single Cell (Intra) |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag17821 Product name: Recombinant human IQGAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 478-827 aa of BC151834 Sequence: QLSSSVTGLTNIEEENCQRYLDELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQEEHERILAIGLINEALDEGDAQKTLQALQIPAAKLEGVLAEVAQHYQDTLIRAKREKAQEIQDESAVLWLDEIQGGIWQSNKDTQEAQKFALGIFAINEAVESGDVGKTLSALRSPDVGLYGVIPECGETYHSDLAEAKKKKLAVGDNNSKWVKHWVKGGYYYYHNLETQEGGWDEPPNFVQNSMQLSREEIQSSISGVTAAYNREQLWLANEGLITRLQARCRGYLVRQEFRSRMNFLKKQIPAITCIQSQWRGYKQKKAYQDRLAYLRSHKDEVVKIQSLARMHQARKRYRD |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Oligo Conjugate |
| 产品类型 | Polyclonal |
| 全称 | MultiPro® 5CFLX Anti-Human IQGAP1 (Polyclonal) |
| 别名 | HUMORFA01, IQGAP1, KIAA0051, p195, SAR1 |
| 计算分子量 | 189 kDa |
| GenBank蛋白编号 | BC151834 |
| 基因名称 | IQGAP1 |
| Gene ID (NCBI) | 8826 |
| ENSEMBL Gene ID | ENSG00000140575 |
| RRID | AB_3673894 |
| 偶联类型 | 5CFLX |
| 完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGTAGCGTCTATAGCCACCCATATAAGAAA |
| 条形码序列 | TAGCGTCTATAGCCA |
| 形式 | Liquid |
| UNIPROT ID | P46940 |
| 储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3. |
| 储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
IQGAP1 is a member of a family of scaffolding proteins that interact with signaling and structural molecules. It localizes to sites of cell-cell contact in epithelial cells and regulates distinct cellular processes including cell adhesion, cell migration, extracellular signals through interacting with numerous protein. Multiple studies have shown that IQGAP1 is up-regulated in many human malignancies, such as lung cancer, ovarian cancer, colon cancer, breast cancer, melanoma and HCC. And IQGAP1 plays a critical role in cancer cell invasion.
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文献和实验Intracellular Immunofluorescent Staining for Flow Cytometry
PBMCs were stimulated with TPA/ionomycin in the presence of monensin for 5 hours. The cells were fixed and permeabilized and intracellularly stained with anti-human CD4, clone RPA-T4, (Cat. No. 17-0049 ) and Alexa Fluor® 488 anti-human IL-17 (Cat. No. 53
Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes
minutes at 60°C with mixing. Insoluble material is pelleted by centrifugation for 15 minutes at 5000xg. Add DABCO (Diazabicyclo(2.2.2.) octane. Merck® #803456) to final 2.5% to the solution as anti-bleaching agent
or overnight at 4°C with agitation. For a mouse monoclonal antibody, a goat anti-mouse HRP-conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat anti-rabbit HRP-conjugated antibody is advisable. Wash the membrane five times
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