- ￥3200
- Proteintech
- CL647-13422
- 2025年10月09日
- FC (Intra)
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PPP3CA protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
CoraLite® Plus 647 Fluorescent Dye
- 适应物种：
human, mouse
- 宿主：
Rabbit
- 应用范围：
FC (Intra)
- 靶点：
PPP3CA
- 抗体英文名：
CoraLite® Plus 647-conjugated PPP3CA Polyclonal antibody
- 抗体名：
CoraLite® Plus 647-conjugated PPP3CA Polyclonal antibody
- 规格：
100ul
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-13422 targets PPP3CA in FC (Intra) applications and shows reactivity with human, mouse samples.
| 经测试应用 | FC (Intra) |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4203 Product name: Recombinant human PPP3CA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3-302 aa of BC025714 Sequence: EPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform |
| 别名 | CALN, CALNA, CALNA1, CAM PRP catalytic subunit, CCN1, CNA, CNA1, PP2BA, PPP2B, PPP3CA |
| 计算分子量 | 59 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC025714 |
| 基因名称 | PPP3CA |
| Gene ID (NCBI) | 5530 |
| RRID | AB_2934886 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q08209 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.
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文献和实验'）2 Fragment（Alexa Fluor® 555 Conjugate）#4413 Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 647 Conjugate）#4414 抗小鼠： Anti-Mouse IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4408 Anti-Mouse IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor
647 34079 SuperSignalâ West Pico Chemiluminescent Substrate Trial Kit 50
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