- ￥5800
- Proteintech
- 80210-13-PBS
- 2025年10月09日
- WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human NANOG protein
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
Nanog
- 抗体英文名：
Nanog Recombinant monoclonal antibody, PBS Only (Capture)
- 抗体名：
Nanog Recombinant monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
80210-13-PBS targets Nanog as part of a matched antibody pair:
MP02157-2: 80210-13-PBS capture and 80210-12-PBS detection (validated in Cytometric bead array, Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag5645 Product name: Recombinant human NANOG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-305 aa of BC160187 Sequence: MSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNNWPKNSNGVTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQTWNNSTWSNQTQNIQSWSNHSWNTQTWCTQSWNNQAWNSPFYNCGEESLQSCMQFQPNSPASDLEAALEAAGEGLNVIQQTTRYFSTPQTMDLFLNYSMNMQPEDV |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Nanog homeobox |
| 别名 | hNanog |
| 计算分子量 | 35 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC160187 |
| 基因名称 | Nanog |
| Gene ID (NCBI) | 79923 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9H9S0 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. There are two kinds of variants that can be recognized by NANOG, one is a normal form (~39 kDa), the other is a post-translation modified form (~48 kDa) (21136380 ). Nanog has two isoforms with molecular weights of 34.4 kDa and 31.9 kDa. (PMID: 21969378)
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文献和实验一、材料及试剂 1、猪瘟单抗纯化抗原 2、兔抗猪IgG酶标抗体 3、猪瘟阳性血清 4、猪瘟阴性血清 1～4均由指定的生物制品所购买。 5、ELISA反应板 6、包被液 碳酸钠 1.50g 碳酸氢钠 2.93g H2O加至 1 000ml pH值9.6 7、PBS液 氯化钠 8.90g 磷酸二氢钾 0.20g 无水磷酸氢二钠 2.13g 加双馏水 1 000ml 8、PBS－吐温洗涤液 PBS液 1 000ml 犊牛血清 5ml 混合即可 9、底物
，重组人FGF-10(产品编号：100-26)需用5mMSodium Phosphate(磷酸钠)，pH7.4溶解，重组IL-13(产品编号：200-13)需用20mMMHCl溶解。(注：即使同一重组细胞因子或蛋白，不同批次的溶解方法也可能有所不同，因此上面的叙述仅供参考。具体应该如何溶解应以相应批次的官方说明书为准)。后续的文章中将对各种溶解液的配制方法进行详细的阐述，望继续关注。 经常有用户会问，为什么会有这么多种溶解方法？ 答：我们知道，蛋白的溶解性与很多因素有关，其中比
Phosphate(磷酸钠)，pH7.4溶解，重组IL-13(产品编号：200-13)需用20mMMHCl溶解。(注：即使同一重组细胞因子或蛋白，不同批次的溶解方法也可能有所不同，因此上面的叙述仅供参考。具体应该如何溶解应以相应批次的官方说明书为准)。后续的文章中将对各种溶解液的配制方法进行详细的阐述，望继续关注。 经常有用户会问，为什么会有这么多种溶解方法? 答：我们知道，蛋白的溶解性与很多因素有关，其中比较重要的是pH值和离子强度。PeproTech的细胞因子或重组蛋白在出厂前均
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