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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human CDH15 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 宿主:
Rabbit
- 应用范围:
WB, IF-P, ELISA
- 靶点:
M-cadherin
- 抗体英文名:
M-cadherin Polyclonal antibody
- 抗体名:
M-cadherin Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | C2C12 cells |
| Positive IF-P detected in | mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29928-1-AP targets M-cadherin in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF-P, ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag25763 Product name: Recombinant human CDH15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 151-450 aa of BC008951 Sequence: AFLQEAFTGRVLEGAVPGTYVTRAEATDADDPETDNAALRFSILQQGSPELFSIDELTGEIRTVQVGLDREVVAVYNLTLQVADMSGDGLTATASAIITLDDINDNAPEFTRDEFFMEAIEAVSGVDVGRLEVEDRDLPGSPNWVARFTILEGDPDGQFTIRTDPKTNEGVLSIVKALDYESCEHYELKVSVQNEAPLQAAALRAERGQAKVRVHVQDTNEPPVFQENPLRTSLAEGAPPGTLVATFSARDPDTEQLQRLSYSKDYDPEDWLQVDAATGRIQTQHVLSPASPFLKGGWYR |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cadherin 15, type 1, M-cadherin (myotubule) |
| 别名 | CDH15, Cadherin 14, Cadherin 15, Cadherin-14, Cadherin-15 |
| 计算分子量 | 89 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC008951 |
| 基因名称 | M-cadherin |
| Gene ID (NCBI) | 1013 |
| RRID | AB_3086192 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55291 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. M-cadherin (muscle cadherin), also known as CDH15 (cadherin-15), is a 124-kDa type I transmembrane glycoprotein that is highly expressed in developing skeletal muscle, satellite cells, and cerebellum (PMID: 9545347; 12052883). It associates with β-catenin and functions as a cell-cell adhesion molecule in a homophilic and specific manner (PMID: 9545347). M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation (PMID: 1840697).
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文献和实验Analysis Project 根据上传的数据类型从下拉菜单中选择新建一个分析项目。2、接着选择之前创建的特定测序项目。3、填写基因组组装的软件。4、成功创建分析项目之后,将看到以下窗口。点击链接进入 IMG/M 进行数据上传。你会获得一个分析项目编号 AP ID,根据提示选择测序数据的质量(完成图或草图)、拓扑(线型或者环状),并选择上传的 fasta 格式序列文件,最后点击 Summit 提交。可以查看数据分析的状态,分析结束后会自动返回 IMG/M 数据库专属的登录号,可以插入到你的文章里以方便读者
参与披网格蛋白小泡组装的一种蛋白质, 分子量为100kDa, 在披网格蛋白小泡组装中与受体的细胞质结构域相互作用, 起衔接作用。有两种类型衔接蛋白, AP1参与反面高尔基体的披网格蛋白小泡的组装, AP2则参与从细胞质膜形成的披网格蛋白的组装。 M6P受体蛋白既存在于反面高尔基体又存在于细胞质膜,所以这种受体既能同AP1作用又能与AP2相互作用。衔接蛋白AP2是一个二聚体,并且是由α衔接蛋白(α链)和β衔接蛋白(β链)两种衔接蛋白组成的异二聚体。 在酵母
-FGF-heparin sepharose complex was transferred in 60 µl 10 mM Tris pH 7.5, to a well of a microtiter plate containing 60 µl of 2« AP assay buffer (2 M diethanolamine, 1 mM MgCl2, 20 mM homoarginine, 12 mM p-nitrophenyl phosphate pH 9.5). The bound
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