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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1, kappa
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
CoraLite® Plus 405 Fluorescent Dye
- 适应物种:
human
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
CD45RA
- 抗体英文名:
CoraLite® Plus 405 Anti-Human CD45RA (F8-11-13)
- 抗体名:
CoraLite® Plus 405 Anti-Human CD45RA (F8-11-13)
- 规格:
25tests/100tests
| 规格: | 25tests | 产品价格: | ¥920.0 |
|---|---|---|---|
| 规格: | 100tests | 产品价格: | ¥2310.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL405-65226 targets CD45RA in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, receptor type, C |
| 别名 | CD45, EC:3.1.3.48, F8-11-13, GP180, L CA |
| 计算分子量 | 1304 aa, 147 kDa |
| GenBank蛋白编号 | BC014239 |
| 基因名称 | CD45 |
| Gene ID (NCBI) | 5788 |
| ENSEMBL Gene ID | ENSG00000081237 |
| RRID | AB_2923725 |
| 偶联类型 | CoraLite® Plus 405 Fluorescent Dye |
| 最大激发/发射波长 | 399 nm / 422 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P08575 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).
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文献和实验*** 849 17,784 19.95*** 802 17,554 20.88*** hsa-miR-200a 517 8,392 15.22*** 667 13,543 19.29*** 722 11,317 14.68*** 500 12,649 24.30*** hsa-miR-218 405 5,654 12.96*** 417 6,527 14.64*** 419 5,764 12
13,045 11,321 −0.15 12,643 10,887
.67144-12.7394-17.1Human1724.1846.7-4.183.785.1633.5-7.5168-2.2Human229.8-2.6829.8-2.7625.1-5.08151-11.7287-9.1Bio-Red1630391.287.1024-14.62522Bio-Red2421.7927018.0-4.44201-14.32980.84* 从总蛋白结果来看RANBOX与Bio-Red 相差为6.45%,而宝灵曼与Bio-Red 较为接近;* 从白蛋白结果分析,宝灵曼与Bio
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