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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1
- 保存条件:
Store at -80°C.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Mouse
- 应用范围:
Sandwich ELISA, Indirect ELISA, Sample test
- 靶点:
VEGFR2/KDR
- 抗体英文名:
VEGFR2/KDR Monoclonal antibody, PBS Only (Detector)
- 抗体名:
VEGFR2/KDR Monoclonal antibody, PBS Only (Detector)
- 规格:
100ug
产品信息
68689-2-PBS targets VEGFR2/KDR as part of a matched antibody pair:
MP50080-1: 68689-1-PBS capture and 68689-2-PBS detection (validated in Sandwich ELISA)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg0218 Product name: Recombinant Human VEGFR2/KDR protein (Myc Tag, His Tag) Source: mammalian cells-derived, pHZ-KIsec Tag: Myc & 6*His Domain: 20-764 aa of NM_002253 Sequence: ASVGLPSVSLDLPRLSIQKDILTIKANTTLQITCRGQRDLDWLWPNNQSGSEQRVEVTECSDGLFCKTLTIPKVIGNDTGAYKCFYRETDLASVIYVYVQDYRSPFIASVSDQHGVVYITENKNKTVVIPCLGSISNLNVSLCARYPEKRFVPDGNRISWDSKKGFTIPSYMISYAGMVFCEAKINDESYQSIMYIVVVVGYRIYDVVLSPSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPSSKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGVTRSDQGLYTCAASSGLMTKKNSTFVRVHEKPFVAFGSGMESLVEATVGERVRIPAKYLGYPPPEIKWYKNGIPLESNHTIKAGHVLTIMEVSERDTGNYTVILTNPISKEKQSHVVSLVVYVPPQIGEKSLISPVDSYQYGTTQTLTCTVYAIPPPHHIHWYWQLEEECANEPSQAVSVTNPYPCEEWRSVEDFQGGNKIEVNKNQFALIEGKNKTVSTLVIQAANVSALYKCEAVNKVGRGERVISFHVTRGPEITLQPDMQPTEQESVSLWCTADRSTFENLTWYKLGPQPLPIHVGELPTPVCKNLDTLWKLNATMFSNSTNDILIMELKNASLQDQGDYVCLAQDRKTKKRHCVVRQLTVLERVAPTITGNLENQTTSIGESIEVSCTASGNPPPQIMWFKDNETLVEDSGIVLKDGNRNLTIRRVRKEDEGLYTCQACSVLGCAKVEAFFIIEGAQEKTNLE |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 亲和力 | KD=3.25 x 10-10M KOff=2.90 x 10-4M KOn=8.91 x 105M |
| 全称 | kinase insert domain receptor (a type III receptor tyrosine kinase) |
| 别名 | KDR, VEGFR2, CD309, EC:2.7.10.1, FLK-1 |
| 计算分子量 | 152 kDa |
| GenBank蛋白编号 | NM_002253 |
| 基因名称 | KDR |
| Gene ID (NCBI) | 3791 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P35968-1 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验1. 杂交瘤阳性克隆的筛选与克隆化 在HAT培养基中生长的杂交瘤细胞,只有少数是分泌预定特异性单克隆抗体的细胞,因此,必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法,筛选出能产生所需单克隆抗体的阳性杂交瘤细胞,并进行克隆扩增。经过全面鉴定其所分泌单克隆抗体的免疫球蛋白类型、亚类、特异性、亲和力、识别抗原的表位及其分子量后,及时进行冻存。2. 单克隆抗体的大量制备 单克隆抗体的大量制备重要采用动物体内诱生法和体外培养法。
过夜。(5)用少许PBS将交联物全部洗出,收集洗出液,加1/20V体积新鲜配制的5mg/ml NaBH4溶液,混匀,室温作用30分钟;再加入3/20V NaBH4溶液,混匀,室温作用1小时(或4℃过夜)。(6)将交联物过Sephadex G200或Sepharose 6B(2.6×50cm)层析纯化,分管收集第一峰。(7)酶结合物质量鉴定:克分子比值测定酶量(mg/ml)=OD403×0.4IgG量(mg/ml)=(OD280-OD403×0.3)×0.62克分子比值(E/P)=酶量×4/IgG量
一、材料及试剂 1、猪瘟单抗纯化抗原 2、兔抗猪IgG酶标抗体 3、猪瘟阳性血清 4、猪瘟阴性血清 1~4均由指定的生物制品所购买。 5、ELISA反应板 6、包被液 碳酸钠 1.50g 碳酸氢钠 2.93g H2O加至 1 000ml pH值9.6 7、PBS液 氯化钠 8.90g 磷酸二氢钾 0.20g 无水磷酸氢二钠 2.13g 加双馏水 1 000ml 8、PBS-吐温洗涤液 PBS液 1 000ml 犊牛血清 5ml 混合即可 9、底物
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