- ￥3580
- Proteintech
- G65190-1-5C
- 2025年10月09日
- Single Cell (Intra), Single Cell
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
2-8°C Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
5CFLX
- 适应物种：
Human
- 宿主：
Mouse
- 应用范围：
Single Cell (Intra), Single Cell
- 靶点：
CD18
- 抗体英文名：
MultiPro® 5CFLX Anti-Human CD18 (TS1/18)
- 抗体名：
MultiPro® 5CFLX Anti-Human CD18 (TS1/18)
- 规格：
10ug
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
| Positive Single Cell detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test |
| SINGLE CELL | <0.5ug/test |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G65190-1-5C targets CD18 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.
| 经测试应用 | Single Cell (Intra), Single Cell |
| 经测试反应性 | Human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Oligo Conjugate |
| 产品类型 | Monoclonal |
| 全称 | MultiPro® 5CFLX Anti-Human CD18 (TS1/18) |
| 别名 | CD18, Integrin beta 2, ITGB2, LAD, LCAMB, LFA 1, MAC 1, MF17, MFI7 |
| 计算分子量 | 85 kDa |
| GenBank蛋白编号 | BC005861 |
| 基因名称 | CD18 |
| Gene ID (NCBI) | 3689 |
| ENSEMBL Gene ID | ENSG00000160255 |
| RRID | AB_3673928 |
| 偶联类型 | 5CFLX |
| 完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGCGTGGTTGGAAGCACCCCATATAAGAAA |
| 条形码序列 | CGTGGTTGGAAGCAC |
| 形式 | Liquid |
| UNIPROT ID | P05107 |
| 储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3. |
| 储存条件 | 2-8°C Stable for one year after shipment. |
背景介绍
CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).
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文献和实验Extraction of DNA using DNAzol® Reagent
mg tissue 1 - 3x107 cells, 0.1 ml liquid sample 2. Centrifugation (optional) 10,000 × g, 10 min 3. DNA Precipitation Lysate
Technovit® 9100 Methyl Methacrylate
analogous to those using paraffin sections. Destabilizing the Basic Solution Fill a chromatography column with 50 g aluminum oxide and allow the Technovit 9100 NEW basis solution (Component No. 1) to flow slowly through it. A column prepared
volume of 35 ml at room temperature. 2) Add the diluted buffy coat on top of 15 ml of Lymphoprep. 3) Centrifuge at 160 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 4) Remove 20 ml of supernatant
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