- ￥600 - 1200
- Proteintech
- Biotin-98262
- 2025年10月09日
- FC
- Rabbit
- recombinant protein
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Biotin
- 适应物种：
recombinant protein
- 宿主：
Rabbit
- 应用范围：
FC
- 靶点：
GGGGS Linker
- 抗体英文名：
Biotin Anti-GGGGS Linker Rabbit Recombinant Antibody
- 抗体名：
Biotin Anti-GGGGS Linker Rabbit Recombinant Antibody
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥600.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1200.0 |
经过测试的应用
| Positive FC detected in | Transfected HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-98262 targets GGGGS Linker in FC applications and shows reactivity with recombinant protein samples.
| 经测试应用 | FC |
| 经测试反应性 | recombinant protein |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | GGGGS Linker |
| 别名 | G4S, G4S link, G4S Linker, GGGGS, GS Linker |
| 基因名称 | |
| Gene ID (NCBI) | 42 |
| 偶联类型 | Biotin |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404).
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文献和实验in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
High-Affinity Immobilization of Proteins Using Biotin- and GST-Based Coupling Strategies
focuses on high-affinity immobilization of protein substrates for affinity and kinetic analyses using biotin/streptavidin interaction and GST/anti-GST-antibody interaction.
The streptavidin-biotin system may be used to synthesize immunoconjugates for targeted delivery of drugs, including therapeutic enzymes. The size of antibody-enzyme conjugates, which is controlled by the extent of biotinylation and molar
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