- ￥800 - 1990
- Proteintech
- CL405-65087
- 2025年10月09日
- FC
- Rat
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2b, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 405 Fluorescent Dye
- 适应物种：
mouse
- 宿主：
Rat
- 应用范围：
FC
- 靶点：
CD45
- 抗体英文名：
CoraLite® Plus 405 Anti-Mouse CD45 (30-F11)
- 抗体名：
CoraLite® Plus 405 Anti-Mouse CD45 (30-F11)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥800.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1990.0 |
经过测试的应用
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL405-65087 targets CD45 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, receptor type, C |
| 别名 | Ptprc, 30-F11, EC:3.1.3.48, LCA, L-CA |
| GenBank蛋白编号 | BC028512 |
| 基因名称 | CD45 |
| Gene ID (NCBI) | 19264 |
| RRID | AB_3083822 |
| 偶联类型 | CoraLite® Plus 405 Fluorescent Dye |
| 最大激发/发射波长 | 399 nm / 422 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P06800 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).
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文献和实验ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
although other techniques can be used. The choice of immobilized antibody binding protein depends upon the species that the antibody was raised in. Protein A binds well to rabbit, cat, human, pig and guinea pig IgG as well as mouse IgG2a and IgG2b . Protein G binds
引进互补的 DNA (cDNA) 文库或者随机 DNA 文库替代模式蛋白（链霉亲和素或 GST) ，那么我们就可以选择出功能蛋白。2. 材料 ( 1 ) pET30a ( Novagen，Darmstadt，Germany)。 ( 2 ) cDNA 文库或随机 DNA 文库。 ( 3 ) 链霉亲和素和 GST 基因。 ( 4 ) RTA 基因。 ( 5 ) 来源于 pCANTAB 5E ( Amersham Biosciences ， fiscataway， NJ
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
slide containing newly expressed proteins (step 14 of protocol 3 ) Secondary antibody (dilute 1:500): Cy3‐labeled anti‐mouse (Jackson ImmunoResearch, cat. no. 115‐165‐071) or Cy3‐labeled
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