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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
WB, IF/ICC, Indirect ELISA
- 靶点:
LGALS3BP
- 抗体英文名:
LGALS3BP Recombinant monoclonal antibody, PBS Only
- 抗体名:
LGALS3BP Recombinant monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
85883-5-PBS targets LGALS3BP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg3302 Product name: Recombinant Human LGALS3BP protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 19-140 aa of NM_005567 Sequence: VNDGDMRLADGGATNQGRVEIFYRGQWGTVCDNLWDLTDASVVCRALGFENATQALGRAAFGQGSGPIMLDEVQCTGTEASLADCKSLGWLKSNCRHERDAGVVCTNETRSTHTLDLSRELS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | lectin, galactoside-binding, soluble, 3 binding protein |
| 别名 | 90K, BTBD17B, Galectin 3 binding protein, Galectin-3-binding protein, Lectin galactoside-binding soluble 3-binding protein |
| 计算分子量 | 585 aa, 65 kDa |
| 观测分子量 | 97, 70 kDa |
| GenBank蛋白编号 | NM_005567 |
| 基因名称 | LGALS3BP |
| Gene ID (NCBI) | 3959 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q08380 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ).
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文献和实验液,加入6ml DMEM完全培养基,37℃孵育过夜,再换液。 7、培养过程中观察细胞生长情况。约2周后可观察到细胞病变(CPE)出现(如用pAdTrack-CMV质粒,由于含GFP,可观察到绿色荧光)。四、重组病毒鉴定 1、转导10-14d后,收集细胞沉淀,加入2ml灭菌的PBS混悬,冻融细胞,离心后收集上清保存于-80℃。 2、取30-50% 步骤1中上清,感染50-70%饱和度的25cm2方瓶中293细胞。2-3d后出现明显细胞病变。 3、感染后3-5d,当1/3-1/2细胞漂浮时
-DNA液,加入6ml DMEM完全培养基,37℃孵育过夜,再换液。 7 培养过程中观察细胞生长情况。约2周后可观察到细胞病变(CPE)出现(如用pAdTrack-CMV质粒,由于含GFP,可观察到绿色荧光)。 四 重组病毒鉴定 1 转导10-14d后,收集细胞沉淀,加入2ml灭菌的PBS混悬,冻融细胞,离心后收集上清保存于-80℃。 2 取30-50% 步骤1中上清,感染50-70%饱和度的25cm2 方瓶中293细胞。2-3d后出现明显细胞
的杂交瘤克隆进行筛选,检测它们的特异性和敏感性。ELISA阳性的克隆可用WB检测,筛选出来的克隆至少还需要亚克隆两次。7. 大规模抗体生产:筛选得到两个克隆最后进行大规模培养(每个克隆1L)或者取腹水(每个克隆5只小鼠)。用蛋白A/G对培养液或腹水进行纯化,平均每个克隆可以得到3-5mg 抗体。8. 注意:每个融合平均可以得到900多个克隆,对于多肽抗原可以得到100个左右的ELISA阳性克隆,重组蛋白抗原可以得到100-150个ELISA阳性克隆。亚克隆实验做的越早,越容易保存克隆。因此,为了保存
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