- ￥5800
- Proteintech
- 67302-3-PBS
- 2025年10月09日
- Cytometric bead array, Indirect ELISA
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Cyclin E protein
- 亚型：
IgG1
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
Cytometric bead array, Indirect ELISA
- 靶点：
Cyclin E
- 抗体英文名：
Cyclin E Monoclonal antibody, PBS Only (Detector)
- 抗体名：
Cyclin E Monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
67302-3-PBS targets Cyclin E as part of a matched antibody pair:
MP50932-1: 67302-2-PBS capture and 67302-3-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag28418 Product name: Recombinant human Cyclin E protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 111-410 aa of BC035498 Sequence: ANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cyclin E1 |
| 别名 | |
| 计算分子量 | 410 aa, 47 kDa |
| GenBank蛋白编号 | BC035498 |
| 基因名称 | Cyclin E1 |
| Gene ID (NCBI) | 898 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P24864 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验Immunodetection of cyclin D1 and D2/D3 using flow cytometry
. It has been tested with antibodies against cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326), cyclin D2/D3 (cat. no. 14711A, clone G107-22) and cyclin E Procedure Prepare the cell type of interest as a single cell suspension and wash once with cold PBS
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
. It has been tested with antibodies against cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326), cyclin D2/D3 (cat. no. 14711A, clone G107-22) and cyclin E, and is based on protocols originally designed by Z. Darzynkiewicz and colleagues. Although FITC-conjugated
过夜。（5）用少许PBS将交联物全部洗出，收集洗出液，加1/20V体积新鲜配制的5mg/ml NaBH4溶液，混匀，室温作用30分钟；再加入3/20V NaBH4溶液，混匀，室温作用1小时（或4℃过夜）。（6）将交联物过Sephadex G200或Sepharose 6B（2.6×50cm）层析纯化，分管收集第一峰。（7）酶结合物质量鉴定：克分子比值测定酶量（mg/ml）=OD403×0.4IgG量（mg/ml）=（OD280-OD403×0.3）×0.62克分子比值（E/P）=酶量×4/IgG量
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