- ￥5800
- Proteintech
- 83752-3-PBS
- 2025年10月09日
- WB, Sandwich ELISA, Indirect ELISA, Sample test
- Rabbit
- human, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human COL1A2 protein
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, rat
- 宿主：
Rabbit
- 应用范围：
WB, Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
Collagen Type I
- 抗体英文名：
Collagen Type I Recombinant monoclonal antibody, PBS Only (Capture)
- 抗体名：
Collagen Type I Recombinant monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
83752-3-PBS targets Collagen Type I as part of a matched antibody pair:
MP00716-3: 83752-3-PBS capture and 83752-4-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag6281 Product name: Recombinant human COL1A2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1017-1366 aa of BC054498 Sequence: RGLPGLKGHNGLQGLPGIAGHHGDQGAPGSVGPAGPRGPAGPSGPAGKDGRTGHPGTVGPAGIRGPQGHQGPAGPPGPPGPPGPPGVSGGGYDFGYDGDFYRADQPRSAPSLRPKDYEVDATLKSLNNQIETLLTPEGSRKNPARTCRDLRLSHPEWSSGYYWIDPNQGCTMDAIKVYCDFSTGETCIRAQPENIPAKNWYRSSKDKKHVWLGETINAGSQFEYNVEGVTSKEMATQLAFMRLLANYASQNITYHCKNSIAYMDEETGNLKKAVILQGSNDVELVAEGNSRFTYTVLVDGCSKKTNEWGKTIIEYKTNKPSRLPFLDIAPLDIGGADQEFFVDIGPVCFK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | collagen, type I, alpha 2 |
| 别名 | COL1, COL1A2, 240665D2, Alpha-2 type I collagen, col I |
| 计算分子量 | 1366 aa, 130 kDa |
| 观测分子量 | 100-160 kDa |
| GenBank蛋白编号 | BC054498 |
| 基因名称 | COL1A2 |
| Gene ID (NCBI) | 1278 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08123 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.
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文献和实验较重要的是pH值和离子强度。PeproTech的细胞因子或重组蛋白在出厂前均经严格测试，说明上所标明的溶解液是能够将该细胞因子或重组蛋白完全溶解的液体。如果您所用的溶解液的pH值和离子强度与说明书中所标明的不符，很多时候会造成细胞因子或重组蛋白不能完全溶解或者根本无法溶解，这样所配得的细胞因子或重组蛋白必然活性不够或丧失。 有不少用户没注意说明书上的描述，而是根据习惯，直接用PBS或培养液(1640或DMEM等)等溶解细胞因子或蛋白的冻干粉。这样做可以吗？ 答：有时可以，要看具体
Dilute to 4% in PBS prior to use Screens for cd-1, size 40 mesh Sigma S07707-5EA Stainless steel grids, 5 per pack
经严格测试，说明上所标明的溶解液是能够将该细胞因子或重组蛋白完全溶解的液体。如果您所用的溶解液的pH值和离子强度与说明书中所标明的不符，很多时候会造成细胞因子或重组蛋白不能完全溶解或者根本无法溶解，这样所配得的细胞因子或重组蛋白必然活性不够或丧失。 有不少用户没注意说明书上的描述，而是根据习惯，直接用PBS或培养液(1640或DMEM等)等溶解细胞因子或蛋白的冻干粉。这样做可以吗? 答：有时可以，要看具体情况。PeproTech的大多数细胞因子或蛋白冻干粉的溶解液不是PBS
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