- ￥600 - 1200
- Proteintech
- CL488-98001
- 2025年10月09日
- FC (Intra)
- Rabbit
- Rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
Rat
- 宿主：
Rabbit
- 应用范围：
FC (Intra)
- 靶点：
IFN-gamma
- 抗体英文名：
CoraLite® Plus 488 Anti-Rat IFN-gamma Rabbit Recombinant Antibody
- 抗体名：
CoraLite® Plus 488 Anti-Rat IFN-gamma Rabbit Recombinant Antibody
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥600.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1200.0 |
经过测试的应用
| Positive FC (Intra) detected in | ConA, PMA and ionomycin stimulated rat splenocytes |
| Positive FC detected in | ConA, PMA and ionomycin stimulated rat splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension |
| Flow Cytometry (FC) | FC : |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-98001 targets IFN-gamma in FC (Intra) applications and shows reactivity with Rat samples.
| 经测试应用 | FC (Intra) |
| 经测试反应性 | Rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interferon gamma |
| 别名 | IFN γ, Ifng, IFNG2, interferon gamma |
| 计算分子量 | 18 kDa |
| GenBank蛋白编号 | NM_138880 |
| 基因名称 | IFN-gamma |
| Gene ID (NCBI) | 25712 |
| RRID | AB_3673356 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01581 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Interferon-gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
overnight. Next, slides were rinsed with water and covered with blocking solution for 2 h at room temperature. Then, after rinsing with water, AlexaFluor-488 labeled anti-mouse antibody conjugate (33 nM in blocking solution) was added to the sample slides
), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)Secondary Antibody250µlIF-IC HCA4414SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)Secondary Antibody250µlIF-IC F4416SAnti-rat IgG (H+L), (Alexa Fluor® 488 Conjugate)Secondary
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