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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
Sandwich ELISA, Indirect ELISA
- 靶点:
PDGFR beta/CD140b
- 抗体英文名:
PDGFR beta/CD140b Recombinant monoclonal antibody, PBS Only (Capture)
- 抗体名:
PDGFR beta/CD140b Recombinant monoclonal antibody, PBS Only (Capture)
- 规格:
100ug
产品信息
82943-6-PBS targets PDGFR beta/CD140b as part of a matched antibody pair:
MP00006-3: 82943-6-PBS capture and 82943-3-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Sandwich ELISA, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg0147 Product name: Recombinant Human PDGFR beta/CD140b protein (Myc Tag, His Tag) Source: mammalian cells-derived, pHZ-KIsec Tag: Myc & 6*His Domain: 33-531 aa of BC032224 Sequence: LVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFFGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPFK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | platelet-derived growth factor receptor, beta polypeptide |
| 别名 | PDGFRB, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140B |
| 计算分子量 | 1106 aa, 124 kDa |
| GenBank蛋白编号 | BC032224 |
| 基因名称 | PDGFR beta |
| Gene ID (NCBI) | 5159 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P09619 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验Immuno-Laser Capture Microdissection
. Single-step PCR is able to amplify fragments of more than 600 bp from both housekeeping genes, e.g., beta-actin, as well as cell-specific messages, e.g., CD4 or CD19, using cDNA derived from less than 500 immunostained, microdissected cells
Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
: Hydrodynamic properties of the complex prepared by a refined hydroxyapatite chromatography in Trition X‐100. Biochim. Biophys. Acta. 462:549‐558. Wilchek, M. and Jakoby, W.B. 1974
/ml,不可振荡。 这个步骤即为溶解步骤,非常重要。 1) 一定要用推荐的溶液重悬(或溶解)冻干粉 用于溶解细胞因子或蛋白的溶液千差万别。此例中的重组人IL-4需用水溶解,而重组人IL-2 (产品编号:200-02)则需用100mM Acetic Acid (醋酸)溶解,重组人TGF-beta1 (产品编号:100-21)需用10mMCitric Acid (柠檬酸),pH3.0溶解,重组人FGF-basic(产品编号:100-18B)需用5mMTris,pH7.6溶解
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