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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2b, kappa
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
CoraLite® Plus 555 Fluorescent Dye
- 适应物种:
human
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
Glycophorin A/CD235a
- 抗体英文名:
CoraLite® Plus 555 Anti-Human Glycophorin A/CD235a (HIR2)
- 抗体名:
CoraLite® Plus 555 Anti-Human Glycophorin A/CD235a (HIR2)
- 规格:
25tests/100tests
| 规格: | 25tests | 产品价格: | ¥1120.0 |
|---|---|---|---|
| 规格: | 100tests | 产品价格: | ¥2800.0 |
经过测试的应用
| Positive FC detected in | human red blood cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-65174 targets Glycophorin A/CD235a in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glycophorin A (MNS blood group) |
| 别名 | Glycophorin A, CD235a, Glycophorin-A, GPA, GPErik |
| 计算分子量 | 150 aa, 16 kDa |
| GenBank蛋白编号 | BC005319 |
| 基因名称 | Glycophorin A |
| Gene ID (NCBI) | 2993 |
| RRID | AB_3084577 |
| 偶联类型 | CoraLite® Plus 555 Fluorescent Dye |
| 最大激发/发射波长 | 554 nm / 570 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P02724 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)
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文献和实验不致在 X 光片上暈開,亦可應用於微陣列 (28),惟基因間佈放相對位置需達 500 mm 以上且放射顯像時間長達三天,顯色分析及螢光偵測則可小於 200 mm。 以玻片及矽晶片為基因承載物,如 Brown 及 Affymetrix 所發展之晶片,則僅能以螢光偵測。免疫酵素顯色、放射線同位素或螢光偵測各有其優劣點,惟目前僅免疫酵素顯色法及螢光偵測法能適用於較高解析之晶片,且能有多重顏色的變化,也適合於一片晶片而多個樣本之研究。 就偵測之靈敏度而言,以放射線同位素較高,而免疫
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