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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human B2M protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IP, ELISA
- 靶点:
Beta-2-Microglobulin
- 抗体英文名:
Beta-2-Microglobulin Recombinant monoclonal antibody
- 抗体名:
Beta-2-Microglobulin Recombinant monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2480.0 |
| 规格: | 150ul | 产品价格: | ¥3280.0 |
经过测试的应用
| Positive WB detected in | A431 cells, HepG2 cells, Raji cells, U-937 cells, Jurkat cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83683-6-RR targets Beta-2-Microglobulin in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag4433 Product name: Recombinant human B2M protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-119 aa of BC032589 Sequence: QRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | beta-2-microglobulin |
| 别名 | B2M, 240660A12, beta 2 microglobulin, beta 2-Microglobulin, Beta-2-microglobulin form pI 5.3 |
| 计算分子量 | 119 aa, 14 kDa |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | BC032589 |
| 基因名称 | B2M |
| Gene ID (NCBI) | 567 |
| ENSEMBL Gene ID | ENSG00000166710 |
| RRID | AB_3671284 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P61769 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.
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文献和实验等)得到表达,然后将此细菌培养物涂在含有氨苄青霉素的选择性培养基上。 重组质粒转化宿主细胞后,还需对转化菌落进行筛选鉴定。利用α互补现象进行筛选是最常用的一种鉴定方法。现在使用的许多载体都具有一段大肠杆菌β半乳糖苷酶的启动子及其编码α肽链的DNA序列,此结构称为lacZ’基因。lacZ’基因编码的α肽链是β半乳糖苷酶的氨基端的短片段(146个氨基酸)。宿主和质粒编码的片段都不具有酶活性,但它们可以通过片段互补的机制形成具有功能活性
相关专题 根据载体的遗传特征筛选重组 子,如α-互补、抗生素基因等。现在使用的许多载体都带有一个大肠杆菌的DNA的短区段,其中有β-半乳糖苷酶基因(lacZ)的调控序列和前146个氨基酸的编码信息。在这个编码区中插入了一个多克隆位点(MCS),它并不破坏读框,但可使少数几个氨基酸插入到β-半乳糖苷酶的氨基端而不影响功能,这种载体适用于可编码β-半乳糖苷酶C端部分序列的宿主细胞。因此,宿主
把亮氨酸自养型载体转入亮氨酸异养型菌株即可在无亮氨酸的培养基中生长。 5.蓝白筛选 蓝白筛选是通过插入失活lacZ基因,破坏重组子与宿主之间的ɑ-互补作用来鉴别重组子与非重组子的筛选方法,是携带lacZ基因的许多载体的筛选优势。这些载体包括M13噬菌体,pUC质粒系列,pEGM质粒系列等。它们的共同特点是载体上携带一段细菌lacZ基因的α肽编码序列,其编码产物为β-半乳糖苷酶的α肽。当无外源DNA插入时,质粒表达α肽。突变
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