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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
WB, IHC, ELISA
- 靶点:
hCG Beta
- 抗体英文名:
hCG Beta Recombinant monoclonal antibody
- 抗体名:
hCG Beta Recombinant monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2480.0 |
| 规格: | 150ul | 产品价格: | ¥3280.0 |
经过测试的应用
| Positive WB detected in | JAR cells, human placenta tisse |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85800-7-RR targets hCG Beta in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg3378 Product name: Recombinant Human hCG Beta protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 21-165 aa of Sequence: SKEPLRPRCRPINATLAVEKEGCPVCITVNTTICAGYCPTMTRVLQGVLPALPQVVCNYRDVRFESIRLPGCPRGVNPVVSYAVALSCQCALCRRSTTDCGGPKDHPLTCDDPRFQDSSSSKAPPPSLPSPSRLPGPSDTPILPQ |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | glycoprotein hormones, alpha polypeptide |
| 别名 | CGB, CGB,hCG, CG beta, CGB3, CGbeta |
| 计算分子量 | 18 kDa |
| 观测分子量 | 20-25 kDa |
| 基因名称 | hCG Alpha |
| Gene ID (NCBI) | 1082 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P0DN86 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta-hCG (beta-human chorionic gonadotropin) is a glycoprotein hormone produced by the placenta during pregnancy. It is a critical marker used in both obstetrics and oncology for various diagnostic and monitoring purposes. hCG is composed of two subunits: an alpha subunit (similar to other glycoprotein hormones like LH, FSH, and TSH) and a unique beta subunit (β-hCG). The beta subunit is the primary focus for diagnostic tests due to its specificity. β-hCG is initially secreted by the syncytiotrophoblast cells surrounding the developing embryo and later by the placenta.
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文献和实验Human chorionic gonadotropin (hCG) has been considered as a primary target molecule for a contraceptive vaccine by the World Health Organization because of its physiological and temporal specificity. hCG is an essential factor
Transforming Growth Factor Beta: A Plasma Tumor Marker
are prostate-specific antigen (PSA), carcinoembryonic antigen (CEA), β-human chorionic gonadotropin (β-HCG), and CA-125. Tumor markers may be specific for certain tumors, such as PSA for prostate cancer, or more generally expressed, such as CEA.
等)得到表达,然后将此细菌培养物涂在含有氨苄青霉素的选择性培养基上。 重组质粒转化宿主细胞后,还需对转化菌落进行筛选鉴定。利用α互补现象进行筛选是最常用的一种鉴定方法。现在使用的许多载体都具有一段大肠杆菌β半乳糖苷酶的启动子及其编码α肽链的DNA序列,此结构称为lacZ’基因。lacZ’基因编码的α肽链是β半乳糖苷酶的氨基端的短片段(146个氨基酸)。宿主和质粒编码的片段都不具有酶活性,但它们可以通过片段互补的机制形成具有功能活性
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