- ￥1140 - 2860
- Proteintech
- CR-65198
- 2025年10月09日
- FC
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Cardinal Red™ Fluorescent Dye
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
CD21
- 抗体英文名：
Cardinal Red™ Anti-Human CD21 (BU32)
- 抗体名：
Cardinal Red™ Anti-Human CD21 (BU32)
- 规格：
25tests/100tests
| 规格： | 25tests | 产品价格： | ￥1140.0 |
|---|---|---|---|
| 规格： | 100tests | 产品价格： | ￥2860.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CR-65198 targets CD21 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | complement component (3d/Epstein Barr virus) receptor 2 |
| 别名 | BU32, C3DR, Complement C3d receptor, Complement receptor type 2, CR2 |
| 计算分子量 | 1092 aa, 119 kDa |
| GenBank蛋白编号 | BC136394 |
| 基因名称 | CD21 |
| Gene ID (NCBI) | 1380 |
| ENSEMBL Gene ID | ENSG00000117322 |
| RRID | AB_3084979 |
| 偶联类型 | Cardinal Red™ Fluorescent Dye |
| 最大激发/发射波长 | 592 nm / 611 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P20023 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.
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文献和实验and then centrifuge at 14,000 RPM for 3 minutes. Save 20µl of the elutes as (Flag-Input). Next, take the remaining ~80µl of elute to clean tubes containing 40µl of EZVIEW™ Red anti-Flag M2 affinity gel beads (Sigma™) which have been pre-treated with a wash in 250µl
网络 三、Ⅱ型补体受体（CD21） Ⅱ型补体受体（CR2）按白细胞分化抗原归类为CD21。CD2为分子量140kDa的单链糖蛋白，主要分布于B细胞、单核细胞、某些T细胞、咽上皮细胞及淋巴结滤泡树突状细胞上。其配体C3bg、C3d和C3bi中的C3d部分。CR2也是EB病毒的受体，CR2与C3d结合的部位和同EB病毒结合的部位
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P标记。二者的区别：33P探针杂交图象质量很高，但是价格昂贵，而且灵敏度低；32P却非常适于低丰度基因表达的检测。近年来，Clontech公司不断推出新研究热点的cDNA Array,您可以通过其公司网址上网查询有关资料，也可以向ebiotrade.com客户服务部索取。Atlas™ Human cDNA Expression Array Cat#7740-1人类功能基因组研究的有力工具包括许多在不同生命过程中起关键作用的基因结果信息量大588种人类cDNA点于一带正电的尼龙膜上，另外还包括9个管家
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