- ￥810 - 2020
- Proteintech
- CL750-65055
- 2025年10月09日
- FC
- Rat
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2b, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 750 Fluorescent Dye
- 适应物种：
human, mouse
- 宿主：
Rat
- 应用范围：
FC
- 靶点：
CD11b
- 抗体英文名：
CoraLite® Plus 750 Anti-Mouse CD11b (M1/70)
- 抗体名：
CoraLite® Plus 750 Anti-Mouse CD11b (M1/70)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥810.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥2020.0 |
经过测试的应用
| Positive FC detected in | mouse bone marrow cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65055 targets CD11b in FC applications and shows reactivity with human, mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | human, mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | integrin alpha M |
| 别名 | CR3, CR3A, integrin alpha M, Itgam, Ly 40 |
| 计算分子量 | 127 kDa |
| GenBank蛋白编号 | BC156991 |
| 基因名称 | CD11b |
| Gene ID (NCBI) | 16409 |
| RRID | AB_2935095 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | E9Q604 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
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文献和实验. no.Q33140) or run on agarose E-gels. After quantification, use 750 ng of total RNA for first strand synthesis using Superscript® III RT kit and QPCR analysis performed using SYBR® GreenER™ qPCR Super Mix
，2000rpm离心10分钟，收集细胞，PBS洗2次，加70%的乙醇混匀，4℃，固定一小时，2000rpm离心10分钟，弃上清，PBS洗2次，加20l PI室温避光放置15分钟，FACS测定。二、实验结果1、抗CD20 ScFv 噬菌体显示文库建立、筛选及鉴定CD20 ScFv噬菌体抗体库的大小约为3x106，即1微克DNA转化后获得106 个克隆。第一次panning前的文库大小为106，经5轮panning后，获得一均一文库，文库的大小约为107。利用ELISA方法，以Daudi细胞为抗原，随机
经750g、离心10min后留上清，以9000g离心20min后留沉淀，重新悬浮后以9000g再离心20min，弃上清得线粒体.全部操作于4℃进行。 (2)培养细胞提取线粒体。细胞弃去培养液后，用细胞收集液作用2到3分钟，用scraper，将细胞收集起来，6000g，离心15分钟。弃上清，之后加细胞匀浆液，用匀浆器或手动匀浆管匀浆，将装有匀浆液的离心管配平后放入普通离心机，以2500rpm，离心15分钟，缓缓取上清液，移入高速离心管中，保存于有冰块的烧杯中。以17000rpm离心20分钟，弃上清
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