- ￥1500 - 3280
- Proteintech
- 82835-3-RR
- 2025年10月09日
- WB, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 靶点：
Phospho-EIF2AK4 (Thr899)
- 抗体英文名：
Phospho-EIF2AK4 (Thr899) Recombinant monoclonal antibody
- 抗体名：
Phospho-EIF2AK4 (Thr899) Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | Calyculin A treated HeLa cells, Calyculin A treated NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82835-3-RR targets Phospho-EIF2AK4 (Thr899) in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA |
| 经测试反应性 | human, mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 2 alpha kinase 4 |
| 别名 | EIF2AK4 (Thr899), EIF2AK4 Thr899, GCN2 (phospho T899), GCN2 (Thr899), p EIF2AK4 |
| 计算分子量 | 187 kDa |
| 观测分子量 | 187 kDa |
| GenBank蛋白编号 | BC009350 |
| 基因名称 | EIF2AK4 |
| Gene ID (NCBI) | 440275 |
| RRID | AB_3670570 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9P2K8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4) belongs to a family of protein kinases that phosphorylate the α subunit of EIF2 (eukaryotic translation initiation factor 2) in response to various stress stimuli. Phosphorylation of EIF2A in response to EIF2AK4 activation subsequently increases translation of specific mRNAs that are important for stress remediation such as ATF4 (activating transcription factor 4). (PMID: 26986695)
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
defined by their ability to form plaques on E. coli P2 lysogens.Commonly used bacterial strainsC600- F-, e14, mcrA, thr-1 supE44, thi-1, leuB6, lacY1, tonA21, l--for plating lambda (gt10) libraries, grows well in L broth, 2x TY, plate on NZYDT+Mg.-Huynh
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
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