- ￥2800
- Proteintech
- CL488-81350
- 2025年10月09日
- IF/ICC, FC (Intra)
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human TDP-43 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
Human, Mouse, Rat
- 宿主：
Rabbit
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
TDP-43 (C-terminal)
- 抗体英文名：
CoraLite® Plus 488-conjugated TDP-43 (C-terminal) Recombinant monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated TDP-43 (C-terminal) Recombinant monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-81350 targets TDP-43 (C-terminal) in IF/ICC, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | Human, Mouse, Rat |
| 免疫原 |
CatNo: Ag4003 Product name: Recombinant human TDP-43 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 261-414 aa of BC001487 Sequence: PKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQGNMQREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSGFNGGFGSSMDSKSSGWGM |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | TAR DNA binding protein |
| 别名 | ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43 |
| 计算分子量 | 43 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC001487 |
| 基因名称 | TDP-43 |
| Gene ID (NCBI) | 23435 |
| RRID | AB_3084500 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13148 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).
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文献和实验3vetdXhbyBVdnXKd85PvkJz/p6enX+LJGxiADv+effz7RPT/6Bi8nkD8C+2CPrOQfCDryEiA+EFkCstAvkJO45E3gF1npz+GlNHhWQTMccILg066YzbBxb4WvDexz7yHPRRdd6Pky14DnAC8ZKm6XfPRd7hVkRpPMPUEZkGm+9PCVg2Pua54T5+irBBpWXMtBwtGSQyQhudxXLFzDpMsf/OAH/jygfUL/g2QTjzLDpE3qCg5gyDOAe5c43A+c456mLcI
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色（可选） 1. 将细胞重悬在 0.5 ml DNA 染色液中（例如 DRAQ5® #4084）。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗： 抗兔： Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4412 Anti-Rabbit IgG（H+L）， F（ab
增殖及计数分析 02 细胞鉴定和免疫分型 荧光标记的CD抗体（Abs）被广泛用于流式细胞术进行细胞识别和免疫分型，至少有15种人类T细胞亚型被描述出来（例如，CD4+，CD8+等）。Incucyte®利用相同的原理，对标记方法进行改进： 将抗CD抗体结合到同型匹配的带有荧光标签的Fc区域靶向Fab片段，便可通过简单的一步混合来完成标记。在检测中使用一种非干扰性的背景荧光抑制染料，以改善488 nm的荧光信号，保护细胞的同时提供更灵活的分析方案。 03 细胞激活和分化 免疫
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