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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human CDX2 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
CoraLite® Plus 488 Fluorescent Dye
- 适应物种:
human, mouse
- 宿主:
Rabbit
- 应用范围:
IF/ICC, FC (Intra)
- 靶点:
CDX2
- 抗体英文名:
CoraLite® Plus 488-conjugated CDX2 Recombinant monoclonal antibody
- 抗体名:
CoraLite® Plus 488-conjugated CDX2 Recombinant monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive IF/ICC detected in | Caco-2 cells, HepG2 cells |
| Positive FC (Intra) detected in | Caco-2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82659 targets CDX2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag17310 Product name: Recombinant human CDX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-180 aa of BC014461 Sequence: MYVSYLLDKDVSMYPSSVRHSGGLNLAPQNFVSPPQYPDYGGYHVAAAAAAAANLDSAQSPGPSWPAAYGAPLREDWNGYAPGGAAAAANAVAHGLNGGSPAAAMGYSSPADYHPHHHPHHHPHHPAAAPSCASGLLQTLNPGPPGPAATAAAEQLSPGGQRRNLCEWMRKPAQQSLGSQ |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | caudal type homeobox 2 |
| 别名 | CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2, Caudal type homeobox 2 |
| 计算分子量 | 313 aa, 34 kDa |
| 观测分子量 | 33-40 kDa |
| GenBank蛋白编号 | BC014461 |
| 基因名称 | CDX2 |
| Gene ID (NCBI) | 1045 |
| RRID | AB_3673070 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q99626 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.
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文献和实验细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色(可选) 1. 将细胞重悬在 0.5 ml DNA 染色液中(例如 DRAQ5® #4084)。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗: 抗兔: Anti-Rabbit IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 488 Conjugate)#4412 Anti-Rabbit IgG(H+L), F(ab
增殖及计数分析 02 细胞鉴定和免疫分型 荧光标记的CD抗体(Abs)被广泛用于流式细胞术进行细胞识别和免疫分型,至少有15种人类T细胞亚型被描述出来(例如,CD4+,CD8+等)。Incucyte®利用相同的原理,对标记方法进行改进: 将抗CD抗体结合到同型匹配的带有荧光标签的Fc区域靶向Fab片段,便可通过简单的一步混合来完成标记。在检测中使用一种非干扰性的背景荧光抑制染料,以改善488 nm的荧光信号,保护细胞的同时提供更灵活的分析方案。 03 细胞激活和分化 免疫
化钙,(问题所在),吹打均匀,孵育1~10min,测定荧光即Fmax值。 (4)加入EGTA,20μl(钙螯合剂),孵育1~10min,激发波长488nm测定荧光,即Fmin值。 这个过程中的氯化钙的作用如何, 请高人指点, 谢谢。 因为正常血浆中Ca2+浓度是1mM,细胞外液的Ca2+对细胞内Ca2+有影响。加0.1%triton是增加膜通透性,使细胞外Ca2+进入细胞内,作为最大值。加EGTA是为了螯合Ca2+,作为最小值.生理环境中细胞内钙离子浓度远低于细胞外液(大约1:10000
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