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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human MGMT protein
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
Cytometric bead array, Indirect ELISA
- 靶点:
MGMT
- 抗体英文名:
MGMT Recombinant antibody, PBS Only (Detector)
- 抗体名:
MGMT Recombinant antibody, PBS Only (Detector)
- 规格:
100ug
产品信息
89933-2-PBS targets MGMT in Cytometric bead array, Indirect ELISA applications and shows reactivity with human samples.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag9996 Product name: Recombinant human MGMT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-207 aa of BC000824 Sequence: MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQCTAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPKAARAVGGAMRGNPVPILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHEGHRLGKPGLGGSSGLAGAWLKGAGATSGSPPAGRN |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | O-6-methylguanine-DNA methyltransferase |
| 别名 | 6-O-methylguanine-DNA methyltransferase, EC:2.1.1.63, Methylated-DNA--protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase |
| 计算分子量 | 22 kDa |
| GenBank蛋白编号 | BC000824 |
| 基因名称 | MGMT |
| Gene ID (NCBI) | 4255 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P16455 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验DETECTION OF RECOMBINANT PROTEINS BY ELISA
. Wait 2 hours at room temperature or overnight at 4°C. Plates coated with the 52kD Ro/SSA recombinant protein can be stored at -70°C for 2 months. The stability of other molecules may vary. Rinse the plates 5 times with PBS-Tween washing
in immunoassays. In respect to surface blocking, different types of nonspecific interactions can show up as increased background. A common problem is that the detector antibody can nonspecifically bind either to a non-optimally blocked surface or, in case
cyanine dye (Cy5―NHS; Amersham Pharmacia Biotech). These reagents react with primary amino groups in the antigen molecule, and may impair the antigen’s ability to react with the antibody after labeling. Phosphorylation of recombinant antibodies
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