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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
CoraLite® Plus 750 Fluorescent Dye
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
FC
- 靶点:
CD22
- 抗体英文名:
CoraLite® Plus 750 Anti-Human CD22 Rabbit Recombinant Antibody
- 抗体名:
CoraLite® Plus 750 Anti-Human CD22 Rabbit Recombinant Antibody
- 规格:
25tests/100tests
| 规格: | 25tests | 产品价格: | ¥400.0 |
|---|---|---|---|
| 规格: | 100tests | 产品价格: | ¥800.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-98092 targets CD22 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD22 molecule |
| 别名 | 241315A1, B cell receptor CD22, B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule |
| 计算分子量 | 847 aa, 95 kDa |
| GenBank蛋白编号 | BC109306 |
| 基因名称 | CD22 |
| Gene ID (NCBI) | 933 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P20273 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.
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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
4 Antibody 22. Rabbit Anti-SeV Antibody 23. Alexa Fluor® 488 Goat Anti-Mouse IgG (H L) Antibody 24. Alexa Fluor® 594 Goat Anti-Mouse IgG (H L) Antibody 25. Alexa Fluor® 488 Goat Anti-Rabbit IgG (H L) Antibody 26. Alexa Fluor® 594
x stock Tris/ Glycine SDS buffer were from National Diagnostics, (Hull, UK).eNOS (NOS 3) affinity purified rabbit polyclonal antibody was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, Calafornia, USA).Goat Anti-Rabbit horseradish peroxidae
protein depends upon the species that the antibody was raised in. Protein A binds well to rabbit, cat, human, pig and guinea pig IgG as well as mouse IgG2a and IgG2b. Protein G binds strongly to IgG from cow, goat, sheep, cow, horse, rabbit and guinea pig
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