- ￥5800
- Proteintech
- 84473-4-PBS
- 2025年10月09日
- Sandwich ELISA, Indirect ELISA, Sample test
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
Neutrophil elastase
- 抗体英文名：
Mouse Neutrophil elastase Recombinant monoclonal antibody, PBS Only (Capture)
- 抗体名：
Mouse Neutrophil elastase Recombinant monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
84473-4-PBS targets Neutrophil elastase as part of a matched antibody pair:
MP01355-4: 84473-4-PBS capture and 84473-5-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg2126 Product name: Recombinant Mouse Neutrophil elastase protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 27-260 aa of NM_015779.2 Sequence: SEIVGGRPARPHAWPFMASLQRRGGHFCGATLIARNFVMSAAHCVNGLNFRSVQVVLGAHDLRRQERTRQTFSVQRIFENGFDPSQLLNDIVIIQLNGSATINANVQVAQLPAQGQGVGDRTPCLAMGWGRLGTNRPSPSVLQELNVTVVTNMCRRRVNVCTLVPRRQAGICFGDSGGPLVCNNLVQGIDSFIRGGCGSGLYPDAFAPVAEFADWINSIIRSHNDHLLTHPKDR |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | elastase 2, neutrophil |
| 别名 | EC:3.4.21.37, Ela2, Elane, Elastase-2, Leukocyte elastase |
| 计算分子量 | 29KD |
| GenBank蛋白编号 | NM_015779.2 |
| 基因名称 | Ela2 |
| Gene ID (NCBI) | 50701 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q3UP87 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验Chromosome Conformation Capture (3C) (alternate technique)
Conformation Capture) technique generates a population average measurement of juxtaposition frequency between any two genomic loci, thus providing information on their relative proximity in the nucleus (Dekker et al ., 2002). Cells are fixed with formaldehyde
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
，而是根据习惯，直接用PBS或培养液(1640或DMEM等)等溶解细胞因子或蛋白的冻干粉。这样做可以吗? 答：有时可以，要看具体情况。PeproTech的大多数细胞因子或蛋白冻干粉的溶解液不是PBS，此时千万不能用PBS或培养液直接来溶解，具体原因在上面已经叙述过。而有部分细胞因子，如重组人KGF(产品编号：100-19)和重组人FGF-23(产品编号：100-52)等，说明书上的溶解液即为1x PBS，此时用PBS溶解完全没问题，那么用培养液溶解也是可以的，不过最好还是用先用PBS溶解，然后再用培养
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