- ￥480 - 950
- Proteintech
- CLV510-65612
- 2026年05月27日
- FC
- Mouse
- human, non-human primates
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- 文献和实验
- 技术资料
- 亚型：
IgG2a
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLux Violet 510 Fluorescent Dye
- 适应物种：
human, non-human primates
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
CD16
- 抗体英文名：
CoraLux Violet 510 Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody
- 抗体名：
CoraLux Violet 510 Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody
- 规格：
25tests/100tests
| 规格： | 25tests | 产品价格： | ￥480.0 |
|---|---|---|---|
| 规格： | 100tests | 产品价格： | ￥950.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CLV510-65612 targets CD16 in FC applications and shows reactivity with human, non-human primates samples.
| 经测试应用 | FC |
| 经测试反应性 | human, non-human primates |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
| 别名 | 3G8, CD16A, CD16a antigen, CD16-II, Fc gamma RIII |
| 计算分子量 | 254 aa, 29 kDa |
| GenBank蛋白编号 | BC017865 |
| 基因名称 | CD16a |
| Gene ID (NCBI) | 2214 |
| ENSEMBL Gene ID | ENSG00000203747 |
| 偶联类型 | CoraLux Violet 510 Fluorescent Dye |
| 最大激发/发射波长 | 410 nm / 501 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08637 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).
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文献和实验、ionomycin和Brefeldin A，其中前两者协同激活细胞，后者抑制蛋白转运，从而有效增强胞内细胞因子的积累与检测信号。 03 封闭剂 单核细胞、巨噬细胞和B细胞表面高表达Fc受体，易非特异性结合抗体的Fc段，导致假阳性信号。为解决此问题，菲恩生物提供Purified Anti-Mouse CD16/32 Antibody（FNab30106）用于小鼠样本封闭，；Human Fc Receptor Blocking reagent（K128）用于人样本封闭。关键在于必须选择与样本
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Anti-Human CD16 Antibody FNab10997 死活染料 Calcein AM/PI活死细胞双染试剂盒 FNCK022 FineTest® Red 780(死活染料) K121 FineTest®Violet 510（死活染料） K124 FineTest®Blue 516（死活染料） K129
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