- ￥1000 - 2490
- Proteintech
- CP-65104
- 2025年10月09日
- FC
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2b, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
PerCP Fluorescent Dye
- 适应物种：
Mouse
- 宿主：
Rat
- 应用范围：
FC
- 靶点：
CD4
- 抗体英文名：
PerCP Anti-Mouse CD4 (GK1.5)
- 抗体名：
PerCP Anti-Mouse CD4 (GK1.5)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥1000.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥2490.0 |
经过测试的应用
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CP-65104 targets CD4 in FC applications and shows reactivity with Mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | Mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rat / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD4 antigen |
| 别名 | Cd4, CD4 antigen, L3T4, Ly 4 |
| GenBank蛋白编号 | BC039137 |
| 基因名称 | Cd4 |
| Gene ID (NCBI) | 12504 |
| RRID | AB_3673812 |
| 偶联类型 | PerCP Fluorescent Dye |
| 最大激发/发射波长 | 482 nm / 677 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P06332 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
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文献和实验The Mouse as Model System to Study Host‐Pathogen Interactions in Influenza A Infections
Abstract Table of Contents Materials Figures Literature Cited Abstract The mouse
Intracellular Cytokine Staining Protocol
)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinJES6-5H4IL-4mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinBVD6-24G2IL-6mouse
的功能决定的，所以首先需要注意两点： 流式细胞仪有哪些激光。比如标配的FACSCalibur只有一根488nm的激光，能做FL1、FL2、FL3三个荧光通道/三个颜色，而选配的Calibur (FACSCalibur的简称)配有488nm和635nm两根激光，可以检测FL1-FL4四个通道/4个颜色。 不同型号的流式细胞仪的同样的一个通道检测荧光素的能力是不一样的，比如Calibur的FL3（第3通道）能检测PE-Texas Red, PE-Cy5, PerCP
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