- ￥1500 - 3280
- Proteintech
- 84584-5-RR
- 2025年10月09日
- WB, IHC, IF/ICC, FC (Intra), ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human BCAP31 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), ELISA
- 靶点：
BAP31
- 抗体英文名：
BAP31 Recombinant monoclonal antibody
- 抗体名：
BAP31 Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells, MCF-7 cells, THP-1 cells, mouse liver tissue, rat liver tisssue |
| Positive IHC detected in | human colon cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84584-5-RR targets BAP31 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1668 Product name: Recombinant human BCAP31 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-246 aa of BC014323 Sequence: MSLQWTAVATFLYAEVFVVLLLCIPFISPKRWQKIFKSRLVELLVSYGNTFFVVLIVILVLLVIDAVREIREYDDVTEKVNLQNNPGAMEHFHMKLFRAQRNLYIAGFSLLLSFLLRRLVTLISQQATLLASNEAFKKQAESASEAAKKYMEENDQLKKGAAVDGGKLDVGNAEVKLEEENRSLKADLQKLKDELASTKQKLEKAENQVLAMRKQSEGLTKEYDRLLEEHAKLQAAVDGPMDKKEE |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | B-cell receptor-associated protein 31 |
| 别名 | BCAP31, BCR-associated protein 31, B-cell receptor-associated protein 31, 6C6-AG tumor-associated antigen, 6C6 AG |
| 计算分子量 | 28 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC014323 |
| 基因名称 | BAP31 |
| Gene ID (NCBI) | 10134 |
| RRID | AB_3672082 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P51572 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BAP31, also known as BCAP31, is a chaperone protein abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded proteins, and their targeting to the ER-associated-degradation (ERAD). CDIP1 and BAP31 interact upon ER stress to regulate the mitochondrial apoptosis pathway(PMID: 24139803). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.
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文献和实验ml）lml，再从鸡的翼下静脉取血0.5--1ml，取出后放入刻度离心管中，再加入2--3ml Alsver液，使总量为4--5ml，混匀并封口，置于4℃冰箱内可供一周内使用，实验时取贮备的鸡血细胞lml，加入4ml85％生理盐水，混匀后以1200rr/min离心5min，去上清液后，最后用0.6mol/L的甘露醇液(或0.6mol/L蔗糖溶液)混匀后以上述离心条件洗涤两次，最后用0.6mol/L的甘露醇液将鸡细胞稀释并调整成每毫升含2×105个。如采用传代培养的骨髓或腹水瘤细胞，可先用0.9％
是没有问题的. 2、表达重组蛋白时，细菌裂解方法都有哪些？ 在表达重组蛋白时，诱导以后跑SDS-PAGE发现表达都很好，但是在裂解细胞时遇到问题。总是不能彻底裂解细胞。 首先我采用超声裂解，可是不管我如何超声总有部分细菌没有裂解。 我的超声条件如下：宁波新芝超声细胞破碎仪，功率400W，超5秒，间隔5秒，重复99次。然后显微镜观察，不彻底时再重复99次。菌体来自200 ml培养液，用20 ml PBS重悬。 然后我又尝试加溶菌酶，首先加至终浓度100 ug/ml，4C半小时菌液不变粘
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