- ￥5800
- Proteintech
- 85802-2-PBS
- 2025年10月09日
- IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA
- Rabbit
- mouse
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA
- 靶点：
CD74
- 抗体英文名：
Mouse CD74 Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名：
Mouse CD74 Recombinant monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
85802-2-PBS targets CD74 as part of a matched antibody pair:
MP02137-1: 85802-1-PBS capture and 85802-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg2716 Product name: Recombinant Mouse Cd74 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 56-279 aa of NM_001042605.1 Sequence: QQQGRLDKLTITSQNLQLESLRMKLPKSAKPVSQMRMATPLLMRPMSMDNMLLGPVKNVTKYGNMTQDHVMHLLTRSGPLEYPQLKGTFPENLKHLKNSMDGVNWKIFESWMKQWLLFEMSKNSLEEKKPTEAPPKVLTKCQEEVSHIPAVYPGAFRPKCDENGNYLPLQCHGSTGYCWCVFPNGTEVPHTKSRGRHNCSEPLDMEDLSSGLGVTRQELGQVTL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) |
| 别名 | Class-II-associated invariant chain peptide, CLIP, H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain, Ii |
| 计算分子量 | 32 kDa |
| GenBank蛋白编号 | NM_001042605.1 |
| 基因名称 | Cd74 |
| Gene ID (NCBI) | 16149 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04441-1 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
CD74, also known as MHC class II invariant chain Ii or HLA-DR-associated invariant chain, is a non-polymorphic type II transmembrane glycoprotein. CD74 is mainly distributed in B cells, monocytes, Langerhans cells, dendritic cells and thymic epithelial cells, and is also expressed in epithelial cells. CD74 plays a key role in MHC class II antigen processing and also serves as a cell surface receptor for the macrophage cytokine MIF. CD74 plays an important role in cardiovascular diseases such as atherosclerosis, ischemic heart disease, and myocardial hypertrophy.(PMID: 15475450; PMID: 27752708; PMID: 38992165; PMID: 36712241).
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验1. 抗原及酶标记抗体 ①抗原：兔抗人IgG(Rabbit Aanti-human IgG，Code No.A0423); ②酶标抗抗体：兔抗鼠IgG-HRP(Rabbit Anti-mouse IgG-HRP，Code No.P0260);均为 DAKO 公司产品，使用时按照说明书要求稀释。 4. 洗涤液(含0.05%Tween 20的PBS)：1LPBS 中加入Tween-20 500μL。 5. 封闭液(含1%牛血
蛋白质，加酶标抗抗体，保温后洗涤，加底物保温30分钟后，加酸或碱终止酶促反应，用目测或光电比色测定抗体含量。三、仪器、原料和试剂仪器聚苯乙烯96孔酶标板、微量移液管、酶联免疫阅读仪、水浴锅。原料单克隆抗体试剂1、 抗原及酶标记抗体①抗原：兔抗人IgG(Rabbit Aanti-human IgG，Code No．A0423)；②酶标抗抗体：兔抗鼠IgG-HRP(Rabbit Anti-mouse IgG-HRP，Code No．P0260)；均为DAKO 公司产品，使用时按照说明书要求稀释。4、 洗涤
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
技术资料暂无技术资料 索取技术资料
