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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human PEX14 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
CoraLite®555 Fluorescent Dye
- 适应物种:
human, mouse, rat, monkey
- 宿主:
Rabbit
- 应用范围:
IF/ICC, FC (Intra)
- 靶点:
PEX14
- 抗体英文名:
CoraLite®555-conjugated PEX14 Polyclonal antibody
- 抗体名:
CoraLite®555-conjugated PEX14 Polyclonal antibody
- 规格:
100ul
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-10594 targets PEX14 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, monkey samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human, mouse, rat, monkey |
| 免疫原 |
CatNo: Ag0932 Product name: Recombinant human PEX14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 77-377 aa of BC006327 Sequence: PSSLGPATQVVPVQPPHLISQPYSPAGSRWRDYGALAIIMAGIAFGFHQLYKKYLLPLILGGREDRKQLERMEAGLSELSGSVAQTVTQLQTTLASVQELLIQQQQKIQELAHELAAAKATTSTNWILESQNINELKSEINSLKGLLLNRRQFPPSPSAPKIPSWQIPVKSPSPSSPAAVNHHSSSDISPVSNESTSSSPGKEGHSPEGSTVTYHLLGPQEEGEGVVDVKGQVRMEVQGEEEKREDKEDEEDEEDDDVSHVDEEDCLGVQREDRRGGDGQINEQVEKLRRPEGASNESERD |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | peroxisomal biogenesis factor 14 |
| 别名 | PTS1 receptor docking protein, Pex14p, PEX, Peroxin-14, Peroxin 14 |
| 计算分子量 | 41 kDa |
| 观测分子量 | 57 kDa |
| GenBank蛋白编号 | BC006327 |
| 基因名称 | PEX14 |
| Gene ID (NCBI) | 5195 |
| RRID | AB_2919590 |
| 偶联类型 | CoraLite®555 Fluorescent Dye |
| 最大激发/发射波长 | 557 nm / 570 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75381 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.
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