- ￥2800
- Proteintech
- CL488-82191-3
- 2026年05月27日
- IF/ICC
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
IF/ICC
- 靶点：
IL-10
- 抗体英文名：
CoraLite® Plus 488-conjugated IL-10 Recombinant monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated IL-10 Recombinant monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | LPS and Brefeldin A treated RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82191-3 targets IL-10 in IF/ICC applications and shows reactivity with mouse samples.
| 经测试应用 | IF/ICC |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg0026 Product name: Recombinant Mouse IL-10 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec Tag: N-6*his Domain: 19-178 aa of NM_010548 Sequence: SRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVEQVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin 10 |
| 别名 | Il10, IL-10,IL10, 240430G12, CSIF, Il 10 |
| 计算分子量 | 21 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | NM_010548 |
| 基因名称 | Il10 |
| Gene ID (NCBI) | 16153 |
| RRID | AB_3673063 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P18893 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
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文献和实验Vybrant® DyeCycle Green and Orange Stains
DyeCycle™ Staining Protocol This basic protocol is optimized using Jurkat cells suspended in complete medium (RPMI/10% fetal bovine serum) and stained with Vybrant® DyeCycle™ stain at 37?C. Remove the Vybrant® DyeCycle™ Green stain
•Streamlined sequence analysis Ligase-mediated(overnight)T4 DNA ligase Original TA Cloning® Kit •≥80% recombinants K2000-10K2030-10 •Blue/white color
非常有效的。 图：单克隆抗体用生物素（A）或 Twin-Strep-tag®（B）标记，两种都可以固定在 Strep-Tactin®TACS 琼脂糖层析柱中。 总而言之，作为灵活的细胞研究工具，Strep-Tactin®TACS 琼脂糖层析柱在细胞分选的应用不仅限于阳选，还可以在阴选中结合多种生物素化抗体（或Twin-Strep-tag®标记的抗体）一起使用。整个细胞分选过程只需 10 分钟，是一种快速有效的细胞阴选方案。 实验中用到的产品列表 Strep-Tactin
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