- ￥1200
- Proteintech
- CL488-81115
- 2025年10月09日
- IF/ICC
- Rabbit
- human, mouse, rat, pig, canine
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human beta actin protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human, mouse, rat, pig, canine
- 宿主：
Rabbit
- 应用范围：
IF/ICC
- 靶点：
Beta Actin
- 抗体英文名：
CoraLite® Plus 488-conjugated Beta Actin Recombinant monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated Beta Actin Recombinant monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-81115 targets Beta Actin in IF/ICC applications and shows reactivity with human, mouse, rat, pig, canine samples.
| 经测试应用 | IF/ICC |
| 经测试反应性 | human, mouse, rat, pig, canine |
| 免疫原 |
CatNo: Ag14521 Product name: Recombinant human beta actin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-50 aa of BC002409 Sequence: MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | actin, beta |
| 别名 | Actin, ACTB, bate actin, β actin, Actin, cytoplasmic 1 |
| 计算分子量 | 375 aa, 42 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC002409 |
| 基因名称 | Beta Actin |
| Gene ID (NCBI) | 60 |
| RRID | AB_2934631 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P60709 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )
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文献和实验per 107 cells/100 mm dish/150 cm2 flask)裂解细胞，收集裂解液至离心管中，在振荡器上混匀4～15min，14000g离心15min（4℃），弃沉淀，用Bradford法或其它蛋白质测定方法测定上清中蛋白浓度以调整上样体积和上样量，进行Western杂交时还需设置内或外参照，通常用beta-actin。 注意： 一般上样20~30 µg已足够，如待检蛋白为低丰度蛋白，可加大上样量至100µg，但电泳条带易拖尾，可制备亚细胞组份或采用更敏感的检测方法。 电泳
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丰富的磷酸化抗体产品 公司具有近千种磷酸化蛋白抗体，产品覆盖18中细胞信号通路。抗体产品被广泛应用于细胞信号转导研究、肿瘤研究、蛋白质组功能研究、新药评价和筛选、临床病理诊断等多个领域。 高质量的抗体定制服务 为满足各种客户的需求，公司向广大的科技工作者们提供丰富的定制服务。包括磷酸化多克隆抗体、一般多肽抗体、单克隆抗体、抗体纯化、鉴定等，为他们的科研提供便捷的服务。 严格
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