- ￥5800
- Proteintech
- 84430-2-PBS
- 2025年10月09日
- WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
SIRP Alpha/CD172a
- 抗体英文名：
Mouse SIRP Alpha/CD172a Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名：
Mouse SIRP Alpha/CD172a Recombinant monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
84430-2-PBS targets SIRP Alpha/CD172a as part of a matched antibody pair:
MP01311-4: 84430-3-PBS capture and 84430-2-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg1520 Product name: Recombinant Mouse SIRP alpha/CD172a protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 32-373 aa of NM_007547.4 Sequence: KELKVTQPEKSVSVAAGDSTVLNCTLTSLLPVGPIRWYRGVGPSRLLIYSFAGEYVPRIRNVSDTTKRNNMDFSIRISNVTPADAGIYYCVKFQKGSSEPDTEIQSGGGTEVYVLAKPSPPEVSGPADRGIPDQKVNFTCKSHGFSPRNITLKWFKDGQELHPLETTVNPSGKNVSYNISSTVRVVLNSMDVNSKVICEVAHITLDRSPLRGIANLSNFIRVSPTVKVTQQSPTSMNQVNLTCRAERFYPEDLQLIWLENGNVSRNDTPKNLTKNTDGTYNYTSLFLVNSSAHREDVVFTCQVKHDQQPAITRNHTVLGFAHSSDQGSMQTFPDNNATHNWN |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | signal-regulatory protein alpha |
| 别名 | 241794D9, Bit, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a |
| 计算分子量 | 56 kDa |
| 观测分子量 | 90-140 kDa |
| GenBank蛋白编号 | NM_007547.4 |
| 基因名称 | Sirpa |
| Gene ID (NCBI) | 19261 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P97797-2 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).
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文献和实验1. 抗原及酶标记抗体 ①抗原：兔抗人IgG(Rabbit Aanti-human IgG，Code No.A0423); ②酶标抗抗体：兔抗鼠IgG-HRP(Rabbit Anti-mouse IgG-HRP，Code No.P0260);均为 DAKO 公司产品，使用时按照说明书要求稀释。 4. 洗涤液(含0.05%Tween 20的PBS)：1LPBS 中加入Tween-20 500μL。 5. 封闭液(含1%牛血
蛋白质，加酶标抗抗体，保温后洗涤，加底物保温30分钟后，加酸或碱终止酶促反应，用目测或光电比色测定抗体含量。三、仪器、原料和试剂仪器聚苯乙烯96孔酶标板、微量移液管、酶联免疫阅读仪、水浴锅。原料单克隆抗体试剂1、 抗原及酶标记抗体①抗原：兔抗人IgG(Rabbit Aanti-human IgG，Code No．A0423)；②酶标抗抗体：兔抗鼠IgG-HRP(Rabbit Anti-mouse IgG-HRP，Code No．P0260)；均为DAKO 公司产品，使用时按照说明书要求稀释。4、 洗涤
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
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