- ￥850 - 2120
- Proteintech
- CR-65267
- 2025年10月09日
- FC
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Cardinal Red™ Fluorescent Dye
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
DPP4/CD26
- 抗体英文名：
Cardinal Red™ Anti-Human DPP4/CD26 (BA5b)
- 抗体名：
Cardinal Red™ Anti-Human DPP4/CD26 (BA5b)
- 规格：
25tests/100tests
| 规格： | 25tests | 产品价格： | ￥850.0 |
|---|---|---|---|
| 规格： | 100tests | 产品价格： | ￥2120.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CR-65267 targets DPP4/CD26 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | dipeptidyl-peptidase 4 |
| 别名 | CD26, BA5b, ADCP-2, ADCP2, ADCP 2 |
| 计算分子量 | 88 kDa |
| GenBank蛋白编号 | BC013329 |
| 基因名称 | DPP4 |
| Gene ID (NCBI) | 1803 |
| RRID | AB_2935237 |
| 偶联类型 | Cardinal Red™ Fluorescent Dye |
| 最大激发/发射波长 | 592 nm / 611 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P27487 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).
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文献和实验in a humid chamber for 1 hour, RT. Blot off antibody, wash 2 times 5 min. each in 1x PBS. Prepare the working dilution of the secondary antibody (Horse anti-mouse IgG-Vector Catalog No. BA2001) in 1.0% BSA/PBS, 2.0% normal horse serum. Prepare
在无血清细胞培养条件下将人 iPS 细胞体外分化为结肠类器官
-1000 移液器上下吹打 20 次以分散类器官沉淀团。 向类器官悬浮液中加入 5 mL 1X PBS，并在 4 °C 下以 700 x g 离心 5 分钟。如果上清液中存在残留的类器官碎片，则再离心 5 分钟。 吸出上清液并用 1 mL 3dGRO™ 类器官冷冻培养基 (SCM301) 重悬沉淀。 用 P-1000 移液器重悬沉淀 5 次，加入冷冻培养基以获得四个胶滴每毫升每管 将冷冻保存管中的细胞转移到冷冻容器（例如 Mr. Frosty）中，然后放入 -80 °C 冰箱中 24-72 小时
1, POU2F2, POU3F1, POU5F1, PRF1, PRTN3, PTGIR, PTPN11, RAC2, RAG1, RARA, RBMS1, RFX1, RGS2, RHOH, RIPK1, RUNX1, RUNX3, RYR1, S100A12, SDS, SERPINB2, SF1, SFTPD, SH2D1A, SLC11A1, SLPI, SPI1, STAT1, STAT2, STAT5B, STAT6, STMN1, SYK, TAL1, TAZ, TBX1, TCF
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