- ￥1350 - 3000
- Proteintech
- 32611-1-AP
- 2025年10月09日
- WB, ELISA
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 靶点：
IL-16
- 抗体英文名：
IL-16 Polyclonal antibody
- 抗体名：
IL-16 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32611-1-AP targets IL-16 in WB, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, ELISA |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg3127 Product name: Recombinant Mouse IL-16 protein (C-rFc) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 1205-1322 aa of NM_010551.3 Sequence: SAASASAASDISVESKEATVCTVTLEKTSAGLGFSLEGGKGSLHGDKPLTINRIFKGTEQGEMVQPGDEILQLAGTAVQGLTRFEAWNVIKALPDGPVTIVIRRTSLQCKQTTASADS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 16 |
| 别名 | Il16, Interleukin-16, Pro-interleukin-16 |
| 计算分子量 | 141 kDa |
| 观测分子量 | 66-72 kDa |
| GenBank蛋白编号 | NM_010551.3 |
| 基因名称 | Il16 |
| Gene ID (NCBI) | 16170 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O54824-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL-16 is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. It is mainly produced by T lymphocytes but also by other immune cells, neuronal cells, fibroblasts and epithelial cells. The signaling process of this cytokine is mediated by CD4. IL-16 induces chemotaxis of CD4+ cells such as lymphocytes, eosinophils, and dendritic cells by ligating CD4 directly at a site distinct from other ligands. Among its multiple functions, IL-16 is a T cell chemoattractant involved in T helper cell inflammatory responses and the regulation of both T cell growth, and responsiveness to regulatory cytokines. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Pro-IL-16 can be detected at about 80 kDa, and cleaved fragments can be deteced at between 40 and 75 kDa.
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文献和实验Using ProHits to Store, Annotate, and Analyze Affinity Purification–Mass Spectrometry (AP‐MS) Data
of the software to analyze AP?MS data. Curr. Protoc. Bioinform. 39:8.16.1?8.16.32. © 2012 by John Wiley & Sons, Inc. Keywords: affinity purification coupled with mass spectrometry; data analysis; virtual machine; statistical models; protein
流速为14～16滴/min。分部收集器收集，紫外监测，或人工收集，以紫外分光光度计分别测定每管280nm OD值，描绘洗脱液峰。 6. 浓缩：将洗脱液的280nm OD上峰段与下峰段各管分别合并，装入透析袋，以PEG浓缩至所需体积。 三、Tris-PO4 离子交换层析法 该法在上述方法的基础上稍加改良，即通过梯度洗脱，可用于血清中IgG和IgM的提取。 材料和试剂 1. DE32或DE52纤维素。 2. 待纯化标本：杂交瘤腹水或培养上清，免疫血清或饱和硫酸铵粗提品。
【原创】Tricine SDS-PAGE凝胶配方（16.5％）
这篇配方是我原发在这儿的怕帖子沉了，搜索了一下就开了一个新主题。另有Tricine SDS-PAGE凝胶配方（10％）的配方，有战友需要话我再发出来 。希望用了这个配方的战友如果效果好的话请跟贴说明，效果不好的话，我尽可能帮助战友们分析一下问题，反正这是我实践过的东西，不应该跑步出来的呀^_^另外Tricine SDS-PAGE16.5％是我见过最浓的Tricine SDS-PAGE的胶了，真没听说过20％Tricine SDS-PAGE胶，如果谁有说一下效果怎么样，是否能分离到几百，甚至几十
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