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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
WB, Indirect ELISA
- 靶点:
IL-21
- 抗体英文名:
IL-21 Recombinant monoclonal antibody, PBS Only
- 抗体名:
IL-21 Recombinant monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
83781-4-PBS targets IL-21 in WB, Indirect ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Product name: HumanKine® recombinant human IL-21 protein Source: -derived, Tag: Domain: of Sequence: |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin 21 |
| 别名 | IL21, IL 21, interleukin 21, Interleukin-21, Za11 |
| 计算分子量 | 162 aa, 19 kDa |
| GenBank蛋白编号 | BC066258 |
| 基因名称 | IL-21 |
| Gene ID (NCBI) | 59067 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9HBE4 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Interleukin 21 (IL-21) is a cytokine produced predominantly by cluster of differentiation 4 (CD4+) T-cells and natural killer T-cells. IL-21 is preliminarily expressed in activated cluster of differentiation 4 (CD4+) T-cells and natural killer (NK) T-cells. IL-21 receptor (IL-21R) is a class I cytokine receptor, which requires dimerization with the indispensable common gamma chain (γc) subunit to bind IL-21. IL-21 plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. IL-21 mediates apoptosis in B-CLL cells through up-regulation of the BIM (BH3 family member) and enhances both direct and antibody-dependent cellular cytotoxicity in these cells.
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文献和实验到7.4 然后加水补充到1000ml,15磅20分高压灭菌 或者滤膜除菌 室温保存免疫学手册上的配方:0.23 g NaH2PO4(anhydrous:1.9mM)1.15 g Na2HPO4(anhydrous:8.1mM)9.00g NaCl(154mM)加水 900 ml 调pH 7.2--7.4 再加水补充到1LPBS的配方:5X PBS stock solution80 gm NaCl2 gm KCl21.72 gm Na2HPO4-7H2O2 gm KH2PO4add to total
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2 O分子量 = 178.05,0.2 mol/L溶液含35.01克/升。 C 4 H 2 O 7 ·H 2 O分子量 = 210.14,0.1 mol/L溶液为21.01克/升。 4. 柠檬酸–氢氧化钠-盐酸缓冲液 使用时可以每升中加入1克克酚,若最后pH值有变化,再用少量50% 氢氧
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