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- Mouse Eif4ebp1重组蛋白表达
- 2025年09月14日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：Q60876
基因名：Eif4ebp1
蛋白名：Eukaryotic translation initiation factor 4E-binding protein 1
蛋白别名：
Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 |
基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
Eif4ebp1相关研究文献：
1. Tumoral EIF4EBP1 regulates the crosstalk between tumor-associated macrophages and tumor cells in MRTK. [38944176]
2. eIF4E-independent translation is largely eIF3d-dependent. [39107322]
3. EIF4EBP1 overexpression is associated with poor survival and disease progression in patients with hepatocellular carcinoma. [25658620]
4. Targeting of EIF4EBP1 by miR-99a-3p affects the functions of B lymphocytes via autophagy and aggravates SLE disease progression. [34668631]
5. BRDT is a novel regulator of eIF4EBP1 in renal cell carcinoma. [33125143]
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文献和实验2. eIF4E-independent translation is largely eIF3d-dependent. [39107322]
3. EIF4EBP1 overexpression is associated with poor survival and disease progression in patients with hepatocellular carcinoma. [25658620]
4. Targeting of EIF4EBP1 by miR-99a-3p affects the functions of B lymphocytes via autophagy and aggravates SLE disease progression. [34668631]
5. BRDT is a novel regulator of eIF4EBP1 in renal cell carcinoma. [33125143]
Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
and exhibit bad planes of anesthesia when heavy. This is also a very inexpensive mouse to use. As an alternate, another strain I have used with considerable success is B6D2F1. These mice are hardy and display hybrid vigor. The Equipment For surgery
PROTOCOL General anesthesia inject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation
Vasectomized males are needed to engender pseudopregnancy in mature female mice. Such pseudopregnant females act as hormonally and physiologically competent recipients for microinjected fertilized one-cell eggs (see Chapter 20 ). Vasectomized
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