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- 湖北省武汉市
- Mouse Pla2g2a重组蛋白表达
- 2025年09月14日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：P31482
基因名：Pla2g2a
蛋白名：Phospholipase A2, membrane associated
蛋白别名：
Phospholipase A2, membrane associated, EC 3.1.1.4, Enhancing factor, EF, GIIC sPLA2, Group IIA phospholipase A2, Phosphatidylcholine 2-acylhydrolase 2A
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 |
基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
Pla2g2a相关研究文献：
1. PLA2G2A(+) cancer-associated fibroblasts mediate pancreatic cancer immune escape via impeding antitumor immune response of CD8(+) cytotoxic T cells. [36796670]
2. High expression of PLA2G2A in fibroblasts plays a crucial role in the early progression of carotid atherosclerosis. [39449149]
3. STUB1-mediated K63-linked ubiquitination of UHRF1 promotes the progression of cholangiocarcinoma by maintaining DNA hypermethylation of PLA2G2A. [39267107]
4. Pla2g2a promotes innate Th2-type immunity lymphocytes to increase B1a cells. [36050343]
5. PLA2G2E-mediated lipid metabolism triggers brain-autonomous neural repair after ischemic stroke. [37490917]
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文献和实验2. High expression of PLA2G2A in fibroblasts plays a crucial role in the early progression of carotid atherosclerosis. [39449149]
3. STUB1-mediated K63-linked ubiquitination of UHRF1 promotes the progression of cholangiocarcinoma by maintaining DNA hypermethylation of PLA2G2A. [39267107]
4. Pla2g2a promotes innate Th2-type immunity lymphocytes to increase B1a cells. [36050343]
5. PLA2G2E-mediated lipid metabolism triggers brain-autonomous neural repair after ischemic stroke. [37490917]
Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
and exhibit bad planes of anesthesia when heavy. This is also a very inexpensive mouse to use. As an alternate, another strain I have used with considerable success is B6D2F1. These mice are hardy and display hybrid vigor. The Equipment For surgery
PROTOCOL General anesthesia inject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation
Vasectomized males are needed to engender pseudopregnancy in mature female mice. Such pseudopregnant females act as hormonally and physiologically competent recipients for microinjected fertilized one-cell eggs (see Chapter 20 ). Vasectomized
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