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- 湖北省武汉市
- Mouse Npc2重组蛋白表达
- 2025年09月14日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：Q9Z0J0
基因名：Npc2
蛋白名：NPC intracellular cholesterol transporter 2
蛋白别名：
NPC intracellular cholesterol transporter 2, Epididymal secretory protein E1, mE1, Niemann Pick type C2 protein homolog
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 |
基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
Npc2相关研究文献：
1. The characteristics and biological significance of NPC2: Mutation and disease. [31843136]
2. Insights into the Molecular Mechanisms of Cholesterol Binding to the NPC1 and NPC2 Proteins. [31098815]
3. Structural Basis of Low-pH-Dependent Lysosomal Cholesterol Egress by NPC1 and NPC2. [32544384]
4. The role of NPC2 gene in glioma was investigated based on bioinformatics analysis. [39160329]
5. Structure and function of the NPC2 protein. [15465422]
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文献和实验2. Insights into the Molecular Mechanisms of Cholesterol Binding to the NPC1 and NPC2 Proteins. [31098815]
3. Structural Basis of Low-pH-Dependent Lysosomal Cholesterol Egress by NPC1 and NPC2. [32544384]
4. The role of NPC2 gene in glioma was investigated based on bioinformatics analysis. [39160329]
5. Structure and function of the NPC2 protein. [15465422]
Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
and exhibit bad planes of anesthesia when heavy. This is also a very inexpensive mouse to use. As an alternate, another strain I have used with considerable success is B6D2F1. These mice are hardy and display hybrid vigor. The Equipment For surgery
PROTOCOL General anesthesia inject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation
Vasectomized males are needed to engender pseudopregnancy in mature female mice. Such pseudopregnant females act as hormonally and physiologically competent recipients for microinjected fertilized one-cell eggs (see Chapter 20 ). Vasectomized
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