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- 湖北省武汉市
- Mouse Crisp2重组蛋白表达
- 2025年09月14日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：P16563
基因名：Crisp2
蛋白名：Cysteine-rich secretory protein 2
蛋白别名：
Cysteine-rich secretory protein 2, CRISP-2, Testis-specific protein TPX-1
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 |
基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
Crisp2相关研究文献：
1. CRISP2 Is a Regulator of Multiple Aspects of Sperm Function and Male Fertility. [30759213]
2. CRISP2, CATSPER1 and PATE1 Expression in Human Asthenozoospermic Semen. [34440724]
3. The fate of porcine sperm CRISP2 from the perinuclear theca before and after in vitro fertilization†. [36054334]
4. Investigating the therapeutic potential of hesperidin targeting CRISP2 in intervertebral disc degeneration and cancer risk mitigation. [39268471]
5. Alpha-1-B glycoprotein (A1BG) inhibits sterol-binding and export by CRISP2. [39433128]
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文献和实验2. CRISP2, CATSPER1 and PATE1 Expression in Human Asthenozoospermic Semen. [34440724]
3. The fate of porcine sperm CRISP2 from the perinuclear theca before and after in vitro fertilization†. [36054334]
4. Investigating the therapeutic potential of hesperidin targeting CRISP2 in intervertebral disc degeneration and cancer risk mitigation. [39268471]
5. Alpha-1-B glycoprotein (A1BG) inhibits sterol-binding and export by CRISP2. [39433128]
Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
and exhibit bad planes of anesthesia when heavy. This is also a very inexpensive mouse to use. As an alternate, another strain I have used with considerable success is B6D2F1. These mice are hardy and display hybrid vigor. The Equipment For surgery
PROTOCOL General anesthesia inject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation
Vasectomized males are needed to engender pseudopregnancy in mature female mice. Such pseudopregnant females act as hormonally and physiologically competent recipients for microinjected fertilized one-cell eggs (see Chapter 20 ). Vasectomized
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