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- 湖北省武汉市
- Mouse H2-Ea重组蛋白表达
- 2025年09月14日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：P14439
基因名：H2-Ea
蛋白名：H-2 class II histocompatibility antigen, E-U alpha chain
蛋白别名：
H-2 class II histocompatibility antigen, E-U alpha chain
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 |
基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
H2-Ea相关研究文献：
1. H2-Ea deficiency is a risk factor for bleomycin-induced lung fibrosis in mice. [15466168]
2. Mouse strains with an active H2-Ea meiotic recombination hot spot exhibit increased levels of H2-Ea-specific DNA breaks in testicular germ cells. [14749381]
3. Expression of the H2-Ea gene is modulated by a polymorphic transcriptional enhancer. [9716646]
4. The impact of H2-DM on humoral immune responses. [11714799]
5. The Forgotten: Identification and Functional Characterization of MHC Class II Molecules H2-Eb2 and RT1-Db2. [26740108]
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文献和实验2. Mouse strains with an active H2-Ea meiotic recombination hot spot exhibit increased levels of H2-Ea-specific DNA breaks in testicular germ cells. [14749381]
3. Expression of the H2-Ea gene is modulated by a polymorphic transcriptional enhancer. [9716646]
4. The impact of H2-DM on humoral immune responses. [11714799]
5. The Forgotten: Identification and Functional Characterization of MHC Class II Molecules H2-Eb2 and RT1-Db2. [26740108]
Detection of Meiotic DNA Breaks in Mouse Testicular Germ Cells
hot spot at the H2-Ea gene. Breaks that were due to meiotic hot spot activity could be distinguished from the background of DNA breaks. This highly sensitive approach could be used to study other biological processes where rare DNA breaks are generated.
Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
and exhibit bad planes of anesthesia when heavy. This is also a very inexpensive mouse to use. As an alternate, another strain I have used with considerable success is B6D2F1. These mice are hardy and display hybrid vigor. The Equipment For surgery
PROTOCOL General anesthesia inject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation
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