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- 湖北省武汉市
- Mouse Hsd17b8重组蛋白表达
- 2025年09月14日
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- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：P50171
基因名：Hsd17b8
蛋白名：(3R)-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.n12) (17-beta-hydroxysteroid dehydrogenase 8) (17-beta-HSD 8) (3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit) (KAR alpha subunit) (3-oxoacyl-[acyl-carrier-protein] reductase) (Estradiol 17-beta-dehydrogenase 8) (EC 1.1.1.62) (Protein Ke6) (Ke-6) (Testosterone 17-beta-dehydrogenase 8) (EC 1.1.1.239)
蛋白别名：
3R, EC 1.1.1.n12, 17-beta-hydroxysteroid dehydrogenase 8, 17-beta-HSD 8, 3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit, KAR alpha subunit, 3-oxoacyl-[acyl-carrier-protein] reductase, Estradiol 17-beta-dehydrogenase 8, EC 1.1.1.62, Protein Ke6, Ke-6, Testosterone 17-beta-dehydrogenase 8, EC 1.1.1.239
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 |
基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
Hsd17b8相关研究文献：
1. Inhibiting HSD17B8 suppresses the cell proliferation caused by PTEN failure. [38811827]
2. The Obesity-Related Metabolic Gene HSD17B8 Protects Against Breast Cancer: High RNA/Protein Expression Means a Better Prognosis. [35184130]
3. 17B-hydroxysteroid dehydrogenases as acyl thioester metabolizing enzymes. [30508570]
4. Estradiol induces type 8 17beta-hydroxysteroid dehydrogenase expression: crosstalk between estrogen receptor alpha and C/EBPbeta. [18852215]
5. Temporal variation in DNA methylation during gonadal development in a reptile with temperature-dependent sex determination†. [35835578]
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文献和实验2. The Obesity-Related Metabolic Gene HSD17B8 Protects Against Breast Cancer: High RNA/Protein Expression Means a Better Prognosis. [35184130]
3. 17B-hydroxysteroid dehydrogenases as acyl thioester metabolizing enzymes. [30508570]
4. Estradiol induces type 8 17beta-hydroxysteroid dehydrogenase expression: crosstalk between estrogen receptor alpha and C/EBPbeta. [18852215]
5. Temporal variation in DNA methylation during gonadal development in a reptile with temperature-dependent sex determination†. [35835578]
Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
and exhibit bad planes of anesthesia when heavy. This is also a very inexpensive mouse to use. As an alternate, another strain I have used with considerable success is B6D2F1. These mice are hardy and display hybrid vigor. The Equipment For surgery
PROTOCOL General anesthesia inject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation
Vasectomized males are needed to engender pseudopregnancy in mature female mice. Such pseudopregnant females act as hormonally and physiologically competent recipients for microinjected fertilized one-cell eggs (see Chapter 20 ). Vasectomized
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