- 询价
- Signalway Antibody
- 63161
- 2025年09月22日
- WB;IHC;IF
- Mouse
- Human;Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant fragment of human BRAF expressed in E. Coli. <br>
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human;Mouse
- 保质期：
12 months
- 抗原来源：
Mouse
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Mouse
- 应用范围：
WB;IHC;IF
- 靶点：
Uniprot：P15056
- 抗体英文名：
BRAF Mouse mAb
- 规格：
50ul/100ul
计算分子量:87kDa
配方:Purified antibody in PBS with 0.05% sodium azide.
应用详情:WB:1/500 - 1/2000
IHC:1/200 - 1/1000
ICC:1/200 - 1/1000
ELISA:1/10000
图片:
Western blot analysis using BRAF mouse mAb against Hela (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
,
Confocal Immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cells using BRAF mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye
,
Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF mouse mAb.
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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