- 询价
- Signalway Antibody
- L35486
- 2025年09月22日
- E, IH, IF, FC
- Goat
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human IgG Fab
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human
- 保质期：
12 months
- 抗原来源：
Goat
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Goat
- 应用范围：
E, IH, IF, FC
- 抗体英文名：
Goat Anti-Human IgG Fab - AF660
- 规格：
50ul/100ul
应用详情:IH (1/100 - 1/1000), IF (1/100 - 1/1000), FC (1/1000 - 1/4000), E (Use at an assay dependent concentration)
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文献和实验Production of Recombinant Human IgG Antibodies in the Baculovirus Expression System
Despite antibody fragments like scFv or Fab are sufficient for various tasks, complete recombinant IgG is sometimes needed. Production of IgG molecules is not generally achievable in E. coli . Insect cells are able to produce IgG and the use
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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